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《Heilongjiang Animal Science and Veterinary Medicine》 2018-01
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Effects of MG132 on maturation of oocytes and development of IVF embryos in buffalo

RUAN Ziyun;SHAO Qiming;PAN Erke;ZHAO Xin;QIN Xiling;SHI Deshun;LU Fenghua;State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources,Guangxi University;  
The aim of the present study was to identify the effects of MG132( Z-Leu-Leu-Leu-CHO) on the in vitro maturation of buffalo oocytes,and in vitro fertilization( IVF) of buffalo embryos. The oocytes were respectively cultured in the maturation medium containing different concentration of MG132( 0,1,2. 5 and 5 μmol/L) for 24 h,then the rate of the first polar body extruding was recorded. The matured oocytes were used for IVF,and the development of IVF embryos was further analyzed. Finally,the expression levels of histone methylation( H3K4me3,H3K9me2 and H3 K9 me3) were examined by immunostaining. The results showed that there were no significant difference in the oocyte maturation rate,cleavage rate and eight-cell rate between the groups( P0. 05). But the morula rate and blastocyst rate were significantly increased by treating with 1 μmol/L MG132( P0. 05). While there was no significant difference in the rate of blastocysts between the 5 μmol/L group and control group( P0. 05). In addition,the results of immunostaining showed that the expression levels of H3K4me3 and H3K9me2 were significantly up-regulated by treating with 1 and 5 μmol/L MG132( P0. 05),respectively. However,there were no significant differencet in the expression level of H3 K9 me3 among all the groups( P0. 05). The results indicated that the expression levels of H3K4me3 could be increased by treating with 1 μmol/L MG132,and then development of IVF embryos also could be promoted.
【Fund】: 国家自然科学基金项目(31560633;31360287);; 广西研究生教育创新计划项目(YCBZ2015004)
【CateGory Index】: S823.83
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