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《Heilongjiang Animal Science and Veterinary Medicine》 2018-01
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Study on the expression of regulatory factors in methylation of nuclear transfer

WANG Wei;FU Maozhong;YI Jun;FANG Donghui;TANG Hui;GAN Jia;LAI Songjia;Sichuan Animal Science Academy;Sichuan Agricultural University;  
The aim of the present study was to analyze the changes in the pattern of the methylation reprogramming after somatic cell transfer to the denucleated oocyte. The expression level changs of 10 important factors involved in methylation reprogramming were studied in five developmental stages of early nuclear transfer embryos( gamophase,S phase,2-cell,8-cell and morula) by fluorescence quantification q PCR. The results showed that the low expression of the demethylation regulatory protein AID and APOBEC1 before the 2-cell stage of the nuclear transfer embryo( SCNT embryo) were responsible for significantly decreased demethylation ability. The over expression of MBD1,Me CP2 and MBD3 in S phase could maintain the high methylation level of the genome. Gene mismatch could be caused by the down regulation of MBD4 gene expression level. The results indicated that the abnormal expression of methylation factors could lead to abnormal methylation of the nuclear transfer embryos,which was the main cause of dysplasia or stillbirth.
【Fund】: “十二五”农村领域国家科技计划项目(2015BAD03B04-3);; 四川省应用基础项目(2015JY0181);; 四川省科技支撑计划项目(2015NZ0020);; 四川省育种攻关项目(2016NYZ0050)
【CateGory Index】: Q78;Q813
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