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《Heilongjiang Animal Science and Veterinary Medicine》 2018-01
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Variability analysis of the VP2 gene hypervariable region in Aleutian mink disease virus

ZHANG Lili;SUN Xinpeng;HAN Xianjie;College of Animal Science and Technology,Qingdao Agriculture University;  
The aim of the present study was to understand the variation of VP2 gene hypervariable region in mink aleutian virus( ADV) in Qingdao. A pair of primers were designed according to the nucleotide sequence of ADV VP2 gene in Genbank. The hypervariable region gene of VP2 was amplified by PCR from ADV genome. Sequence analysis and homology comparison were performed by using the software DNAMAN. The results showed that the length of the amplified six ADV VP2 genes was 226 bp,which was consistent with the intended purpose strip size. Sequence analysis showed that the nucleotide homology of the high variable region of the ADV VP2 gene in the six strains was 92. 9% ~ 100%. The nucleotide homology was 86. 7% ~ 97. 3% compared with the ADV VP2 gene of the domestic and foreign 12 strains. Among them,except 2 strains of the same gene sequence,there were very large mutation in the other 4 strains. And there were also large difference compared with the domestic and foreign strains. The results indicated that the ADV VP2 gene was variable.
【Fund】: 山东省现代农业产业技术体系毛皮动物产业创新团队项目(SDAIT-18-011-12)
【CateGory Index】: S852.65
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