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《Heilongjiang Animal Science and Veterinary Medicine》 2018-01
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Bioinformatics analysis of the MSTN gene sequence in Alxa bactrian camel

YANG Hui;Erdemtu;JIANG Jianqiang;LIU Tuya;DAI Qin;Alatenhuyiga;BAI Long;Inner Mongolia Agricultural University College of Veterinary Medicine,Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease;Veterinary Bureau of Alxa Left Banner;Veterinary Bureau Livestock Board of Inner Mongolia Alxa Left;Inner Mongolia Alxa Barenbieli Animal Safety Supervision Station;Inner Mongolia Etuokeqi Mengxi Town of Agriculture and Animal Husbandry Law Enforcement Team;  
The aim of the present study was to identify the Myostatin( MSTN) gene and analyze the structure and function of MSTN gene in the Alxa bactrian camel. 5 pairs of primers were used to amplify the MSTN gene of Alxa bactrian camel by PCR. The sequences of the products were spliced. The structure,physical and chemical properties of MSTN gene,signal peptide,transmembrane location and subcellular localization of MSTN gene in the Alxa bactrian camel were analyzed by bioinformatics method. The evolution of the MSTN amino acid sequence in Alxa bactrian camel and other species was also discussed. The results showed that the length of MSTN gene was 7. 3 kb. The region of 1-581 bp was the first exon,and the region of 2 384-2 757 bp was the second exon,the region of 4 800-6 737 bp was the third exon of the gene. There was a transmembrane domain in the MSTN protein,the 18 ~ 19 amino acids position was the most likely splicing site,and the peptide segment prior to the sit was the most likely signal peptides. The ratios of α-helix,extended strand,β-turn and random coil in the secondary structures were22. 40%,26. 67%,7. 73% and 43. 20%,respectively. The functional area might be located at the amino acid 278 ~ 375. The MSTN gene was closely related to mammals and primates,and the relationship with the pig was the closest( similarity was 99. 2%). The results indicated that the the protein encoded by MSTN gene in the Alxa bactrian camel was conformed to the structural characteristics of the general TGF-β superfamily,and conformed to the structural characteristics of the known MSTN protein. The molecular evolution distance of the MSTN protein was closed to the pigs,primates and ruminant.
【Fund】: 内蒙古自治区科技计划资助项目(201502069)
【CateGory Index】: S824
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