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《Heilongjiang Animal Science and Veterinary Medicine》 2018-01
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Bioinformatics analysis and promoter prediction on CBD103 gene in Arctic fox

GUO Min;NIU Di;LIU Yanjun;PENG Yongdong;ZHANG Wenxiang;LIU Zhengzhu;FENG Minshan;LI Xianglong;GONG Yuanfang;College of Animal Science and Technology,Hebei Normal University of Science & Technology;  
The aim of the present study was toidentify the Arctic fox CBD103 gene core promoter activity domain,transcription factor binding sites,structure and function of CBD103 protein,and to explore the expression regulation mechanism of the gene. The c DNA sequences of CBD103 gene was obtained from Arctic fox skin tissue by PCR amplification and sequencing. And the bioinformatics methods were used to predict and analyze the resulting sequences.The results showed that the total length of the c DNA sequence was 2 327 bp( including 2071 bp 5' flanking region,204 bp CDs region and 52 bp 3' flanking region).The gene encoded 67 amino acid residues.There was a potential promoter region and a Cp G islandin the Arctic fox CBD103 gene. Some regulatory elementthes( such as TATA box,CAAT box and GC box) and transcription factors( such as Sp1,Ets,Mef-2,ERE and Myc) were also found in the CBD103 gene. The protein encoded by CBD103 was an unstable hydrophobic protein which was located in the nucleus and mitochondria.This protein had a large proportion of random coil,which might lead to a variety of protein conformation. The protein might play a role in signal transduction and transcription. The transmembrane region and signal peptide were also found in the protein. The genetic relationship between the Arctic fox and the Canis lupus familiaris was the most recent. The results indicated that CBD103 gene was a conserved protein among different species. And the genetic relationship between different species was consistent with the biological evolutionand animal taxonomy.
【Fund】: 河北省自然科学基金项目(C2016407114C;2017407040)
【CateGory Index】: S865.23
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