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《Heilongjiang Animal Science and Veterinary Medicine》 2018-01
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The contribution of stable pH value on the improvement of liquid storage of boar semen

XING Yuechao;WANG Shunwei;SUN Meng;WEN Jing;WANG Na;YANG Kang;ZHANG Yuting;YUE Wei;YUE Shunli;ZHOU Jiabo;TAN Jinghe;Key Laboratory of Animal Cell and Genetic Engineering of Heilongjiang Province,College of Life Science,Northeast Agricultural University;  
To study the effect of pH value changes on liquid storage of boar semen,and to find out a suitable pH value for liquid boar semenpreservationwhich could improve the ingredients of liquid storage and could enhance artificial insemination efficiency,the effect of different pH value conditions on liquid storage of boar semen was compared in this experiment. By means ofmonitoring the factors including sperm motility,sperm survival time,acrosome and membrane integrityand other factors,the effect of pH value changes on liquid storage of boar semen was studied and the improvement of liquid storage was attempted. The results showed that the best initial preservation pH was 6.40 when the Androhep was used as storage liquid; andstable pH value could significantly improvesperm motility( P0. 05) on day 3 post semen storage. When 2-morpholinoethanesulfonic acidinstead of 4-hydroxyethylpiperazine ethane sulfonic acid( HEPES) which was contained in the Androhep storage liquid,sperm motilityand survival time were improved significantly( P 0. 05),and meanwhile membrane integrity was maintained betteron day 3 post semen storage,but there was no significant difference in acrosomeintegrity( P0. 05). The results verified that storage liquidwith stable pH value could be more suitable for semen preservation at room temperature.
【Fund】: 国家自然科学基金面上项目(31372311);; 东北农业大学人才基金项目(2012RCB18;2012RCA03)
【CateGory Index】: S828
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