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《Heilongjiang Animal Science and Veterinary Medicine》 2018-01
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Detection of 5 important viruses in semen of boar in scale-premises in Jiangxi by RT-PCR/PCR assays

GONG Wang;SONG Deping;TANG Yuxin;WANG Wangbao;ZHANG Min;LI Hao;ZHANG Fanfan;ZHOU Xinrong;College of Animal Science and Technology,Jiangxi Agricultural University;Agricultural Livestock Fisheries Bureau of Xiushui;  
In order to address possible presence of some important viral agentsin boar semen,polymerase chain reaction( PCR),and/or reverse transcription-PCR( RT-PCR) technologies were used to detect the Porcine reproductive and respiratory syndrome virus( PRRSV),Pseudo rabies disease poison( PRV) and Porcine circovirus type 2( PCV-2),Porcine epidemic diarrhea virus( PEDV) and porcine deltacoronavirus( PDCo V) in 111 boar semen sampled from 3 large-scaled pig farms,referred to as farm A,B,and C,in Jiangxi Province. The results showed that PRRSV,PRV and PCV-2 were detectable in board semen from farm A and B,and PRRSV and PCV-2 farm C and none of the samples from all three farms was positive for PEDV and PDCo V. In all of the 111 semen samples,PCV-2 was the most detectable virus with a positive rate of 74.77%,and followed by PRV( 15.32%) and PRRSV( 9.91%),respectively. Additionally,9.01% and 13.51% co-infection rate of PRRSV with PCV-2 and PCV-2 with PRV were observed in these samples,respectively. The findings of this study indicated that the boar semen could be the reservoirs and dissemination medium for multiple important pathogenic porcine viruses,which might adversely affect the health of pig herds in Jiangxi,China.
【Fund】: 国家自然科学基金项目(31372457);; 江西省科技厅落地计划项目(KJLD13029)
【CateGory Index】: S852.65
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