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《Heilongjiang Animal Science and Veterinary Medicine》 2018-01
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Proliferative characteristics of avian reovirus in the Vero cells

SHEN Wenkang;XIE Zhixun;WANG Sheng;HUANG Li;XIE Liji;HUANG Jiaoling;ZENG Tingting;ZHANG Yanfang;ZHANG Minxiu;LUO Sisi;FAN qing;XIE Zhiqin;DENG Xianwen;College of Animals Science and Technology,Guangxi University;Guangxi Veterinary Research Institue,Guangxi Key Laboratory of Veterinary Biological Technology;  
The aim of the present study was to identify the proliferative characteristics of avian reovirus in green monkey kidney cells( Vero).The cell culture was analyzed by optical microscopy,semi-tissue infection( TCID50) and RT-PCR. The results showed that ARV S1133 could effective proliferation in the Vero cells. Several typical cytopathic( CPE) such as cell disruption,atrophy,aggregation,shedding,edge blur,bigger and even death could be observed on the course of ARV infection in the Vero cells. The gene( 1 248 bp) was successfully amplified from the infected cells by RT-PCR. After infection with Vero cells,the ARV will undergo three periods: latent period,rapid growth period and stabilization period. The virus titer could reach peak( TCID_(50)= 1×10~(-8.46)/0.1 mL) at the stabilization period.
【Fund】: 国家自然科学基金项目(31660715);; 国家“万人计划”领军人才专项(2016-37);; 中国东盟及一带一路南亚国家动物疫病防控技术研发联合实验室建设项目(桂科AB16380003);; 广西自然科学基金项目(2014GXNSFCA118006);; 广西水产畜牧兽医局科技项目(桂渔牧科201452003);; 广西兽医生物技术室开放基金项目(14-045-31-A-2)
【CateGory Index】: S852.65
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