Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Growth Inhibition of High Intensity Focused Ultrasound on Pancreatic Cancer Cell Line Capan-1 in Vitro

LU Run-zhang,GUAN Xiao-jun,ZHAO Jing-hui,et al.(The Institute of Hematology & Oncology of Harbin,Harbin 150010,CHINA)  
Objective To explore the biological effects with different irradiation times or intensity of high intensity focused ultrasound(HIFU) on pancreatic cancer cell line Capan-1 and investigate the relationship between the ultrasonic dosage and therapeutic effects.Methods Cancer cell line Capan-1 was used as the research object and the survival rate was detected by MTT assay in treatment with HIFU.The cell lines in treatment with HIFU were analyzed for apoptosis by DNA ladder and flow cytometer with Annexin V PI staining.Results The results of MTT showed that HIFU significantly inhibited the growth of cell line Capan-1.With the increasing irradiation time and strength,the inhibitory effects increased significantly.The medial lethal dose values of HIFU on Cancer cell line Capan-1 was 269.8 W/cm2(7 s).The results by PCR and flow cytometer showed that irradiation of HIFU on Capan-1 cells induced cell apoptosis with a less dose.Conclusion HIFU could inhibit the growth of cell line Capan-1 and induce apoptosis cells.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved