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《Journal of South China University of Technology(Natural Science Edition)》 1991-01
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STUDIES ON ISOLATION-FUSION TECHNIQUE OF PROTOPLASTS OF THE YEAST SACCHAROMYCES CEREVISIAE Ⅰ. STUDIES ON REGENERATION AND ISOLATION OF PROTOPLASTS IN SACCHAROMYCES CEREVISIAE

Lin Weitie, Tan Fenghui, Yao Ruhua and Chen Lianjiu (Dept. of Food Eng., South China Univ. of Tech.)  
The isolation-fusion technique of protoplasts of microbial cell is a kind of new and developing technique growing on the basis of the cell fusion. In order to achieve distant hybridization, we choose Saccharomyces cerevisiae which is capable of high alcohol productivity and Aspergillus niger which is capable of starch fermentation to study the isolation-fusion technique. Some types of fusant, such as karyoplasts/cytoplasts-protoplasts or karyoplasts /cytoplasts-karyoplasts/cytoplasts are expected. This paper contains the effects of several factors on formation and regeneration of protoplasts. Moreover, bovine serum albumin and polyvinylpyrrolidone (PVP) and precursor of yeast cell wall syntheses are added to increase the regeneration ability of protoplasts. When the formation rates of protoplasts are more than 95%, the highest regeneration rate comes up to 58.6%. In addition, the paper presents tentative isolation of protoplasts in Saccharomyces cerevisiae.
【Fund】: 国家教委资助项目;; 广东省科委资助项目
【CateGory Index】: Q932
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【References】
Chinese Journal Full-text Database 1 Hits
1 LUO Jian-mei,LI Jian-shu,GUO Hao,LIU Feng,LIU Dan,WANG Min (Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China);Optimization of Protoplast Regeneration Medium Composition for Streptomyces gilvosporeus by Response Surface Methodology[J];Food Science;2009-07
【Citations】
Chinese Journal Full-text Database 1 Hits
1 TAN Pei-ying WANG Hua(Institute of Microbiology, Academia Sinica, Beijing);FORMATION, REGENERATION AND INTERGENERIC FUSION OF PROTOPLASTS IN SACCHAROMYCES CEREVISIAE AND CANDIDA UTILIS[J];Mycosystema;1983-02
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Zhang Ming(Department of biological engineering,Anhui Agricultural University,Hefei 230036)Wang Yuanjun;Pan Renru(Department of biology,University of Science&Technology of China,Hefei 230026);An Investigation of the Formation and Regeneration of the Saccharomyces cerevisiae Protoplast[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1996-01
2 Xi Liyan, et al (Institute of Dermatology. Nanjing);Study on L-form Induction of C.albicans[J];Acta Academiae Medicinae Sinicae;1992-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Li Xiaolou, Li Dacui (Sichuan Vocational and Technical College, Suining, Sichuan 629000);The Screening of Fungus Produced by Phytase and the Producing Enzyme Tendency[J];Journal of Northern Sichuan Education College;2005-03
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6 Ge Lan Cheng Shupei (The National Key Laboratory of Pollution Control and Resources Reuse, Department of Environmental Sciences and Engineering Nanjing University, 210093, Nanjing, PRC);DNA CONTENT VARIATION OF PROTOPLAST FUSION PRODUCTS DURING THE PROCESS OF GENETIC SUBSTANCE INTEGRATION BETWEEN INTER KINGDOM CELLS[J];JOURNAL OF NAIJING UNIVERSITY (NATURAL SCIENCES);1997-03
7 Wang Yiping Jia Rong Chen Weiyuan LingYun ( Biology Department of Anhui University, Hefei 230039 ) Xu Huaishu ( College of Marine Life Sciences, Ocean University of Qingdao, Qingdao,266003 );Protoplast Fusion between R. capsulate and R. sphaeroides[J];JOURNAL OF OCEAN UNIVERSITY OF QINGDAO;2000-02
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10 Huang Qinni Liu Jia Song Xiuzhen Yang Xiushan(Department of Biology Capital Normal University, Beijing 100037);Protoplast Fusion between Escherichia coli and Bacillus subtilis[J];Journal of Capital Normal University;2002-01
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