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《Journal of South China University of Technology(Natural Science Edition)》 1991-01
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STUDY ON THE MECHANISM OF SEPARATING YEAST PROTEIN WITH LOW NUCLEIC ACID CONTENT BY SALT TREATMENT

Zong Minhua and Cheng Shugong (Dept. of Food Eng.. South China Univ. of Tech.)  
In this paper the conformation change of yeast protein by different salts are studied by fluorescence spectrum with the result that salt is one of the reason why salt can reduce the nucleic acid content of isolated yeast protein (IYP). The process of removing nucleic acid by solt treatment is analyzed thermodynamically and the effects of ions on water structure are contrasted with that on stability of nucleoprotein. The results negated the hydrophobic mechanism by Damodaran and shows that hydrogen bond is the most important secondary bond stabilizing nucleoprotein, it is not the indirect action of salt on nucleoprotein through that changes the structure of solvent but the direct action of salt on nucleoprotein, especially the action of salt on the main chain of nucleoprotein molecules that causes nucleoprotein to dissociate.
【CateGory Index】: QS20
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Chang-lu1,CHEN Jun-wei1,WEI Xiao-kun2,CHEN Mian-hua1,ZHU Han-chun1(1.College of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China;2.Shanghai Qiuzhiyou Bioscience & Biotechnology Co.,Ltd.,Shanghai 200237,China);RNA purification from Candida tropicalis[J];China Brewing;2007-09
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Zong Minima Chen Shugong(South China Institute of Technology Guangzhou);Study on the Separation of Yeast protein with Low Nucleic Acid Content by Saltiing Out Method[J];Food and Fermentation Industries;1990-02
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 Bian Jing\ Tu Chunyan\ Ouyang Pingkai Department of Biotechnology and Bioengineering, Nanjing University of Chemical Technology, Nanjing, 210009, China;EXTRACTION OF RNA FROM TWO DIFFERENT WASTE YEASTS[J];JOURNAL OF NANJING UNIVERSITY OF CHEMICAL TECHNOLOGY;1998-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 SUN Rong-dan,LIU Yng,ZHANG Hong-lin~*,WANG Zhan-yong~*,QIU Feng~*(Department of Environmental engineering,Liaoning University of Petroleum Chemical Technology,Fushun 113001,China);Study on Extracting Ribonucleic Acid from Discarded Brewers Yeast With Alkali Method and Salt Method[J];Amino Acids & Biotic Resources;2006-03
2 Zhang Shuai (Department of Light Industry & Chemistry, Zhaoqing University, Zhaoqing 526061);Extracting RNA from Brewer’s Yeast by Ammonia Analytical Method[J];Guangzhou Food Science and Technology;2004-04
3 SONG Xu-lu,LI Zhi-dong,XU Heng-gang,ZHANG Hong-lin,WANG Zhan-yong(Environmental Engineering Department,Liaoning University of Petroleum and Chemical Technology,Fushun 113001,China);Study on Extracting RNA from Brewing Yeast by Strong Salt Method[J];Chemistry & Bioengineering;2006-05
4 WANG Zhan-yong,KONG Jun-hao(College of Environmental and Biological Engineering,Liaoning University of Petroleum and Chemical Technology,Fushun 113001,China);Comparison on Thick Salt Method and Thin Alkali Method for Extraction of Ribonucleic Acid from Waste Yeast[J];Chemistry & Bioengineering;2007-02
5 XU Ning,LI Zhi-fu,FENG Wen-hua ¨School of Engineering,Taishan Medical College,Taian271000,China;Extraction of Ribonucleic Acid from Fermentation Was tewater of Brewery[J];Industrial Water & Wastewater;2003-03
6 WANG Zhong li (Shandong Light industry Institute,Ji’nan 250100,China);Natural Seasoning-yeast extract's application and its developing prospect[J];Jiangsu Condiment and Subsidiary Food;2005-01
7 Bian Jing\ Tu Chunyan\ Ouyang Pingkai Department of Biotechnology and Bioengineering, Nanjing University of Chemical Technology, Nanjing, 210009, China;EXTRACTION OF RNA FROM TWO DIFFERENT WASTE YEASTS[J];JOURNAL OF NANJING UNIVERSITY OF CHEMICAL TECHNOLOGY;1998-01
8 DONG Jia-wu(Angel Brand Yeast Co.Ltd.,Yichang,Hubei443003,China );Perplexity and Thought of the Production of Yeast Extract by Waste Beer Yeast in China[J];Liquor-making Science & Technology;2003-03
9 LI Shan and WU Zhen-qiang (Bioscience and Bioengineering College of South China Technical Institute, Guangzhou, Guangdong 510640 , China);Study on RNA Extraction from Waste Yeast by Salt Method[J];Liquor-making Science & Technology;2005-07
10 ;Extracting RNA from Spent Brewer's Yeast by the Combination of High Pressure Homogenization and Saline[J];Liquor-making;2004-01
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 GONG Li,PEI Yi-kun,LI Xiao-ling (School of Chemical & Life Science,Changchun University of Technology,Changchun 130012,China);RNA purification from Saccharomyces cerevisiae[J];Journal of Changchun University of Technology(Natural Science Edition);2009-05
2 HUANG Qiuchan1,WEI Youhuan1,LI Yaoyan2 (1.Department of Chemistry and Biology Engineering,Nanning Teachers College,Longzhou 532400,China;2.College of Agricuture,Guangxi University,Nanning 530005,China);Progress in peptide nucleic acid[J];China Brewing;2008-16
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