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《Journal of South China University of Technology(Natural Science)》 2005-01
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Fermentation and Purification of HBscFv from P. pastoris on Pilot Scale

Xiong Sheng1 Xie Qiu-ling2 Wang Yi-fei1 Deng Ning2 Su Kuan-yuan3(1. Bio-medical Research and Development Center, Jinan Univ., Guangzhou 510632, Guangdong, China; 2. College of Bioscience and Biotechnology, Jinan Univ., Guangzhou 510632, Guangdong, China; 3. Center of Infectious Diseases, the 458th Hospital of PLA, Guangzhou 510602, Guangdong, China)  
In order to produce soluble recombinant human anti-HBsAg single-chain Fv (HBsc Fv) in pilot scale, recombinant P. pastoris excreting HBscFv was inoculated in a 30-L fermentor to carry out a fed-batch culture. HBscFv in the supernatan t was then purified with a two-step procedure, namely, ion-exchange and immuno -affinity chromatography. It is found that, after the culture for 7 days, the c ell density (OD 600) of recombinant P. pastoris and the yield of target protein are respectively 334 and 260 mg/L. After the purification, HBscFv is c ollected with a purity of 95% and a productivity of 171 mg/L. The results of bi oassay demonstrate that the specific activity of HBscFv produced by P. pastori s is (2 52±0 17) μg -1, without significant difference from that prod uced by E. coli..
【Fund】: 国家自然科学基金资助项目 (30 3716 6 1 30 4 0 0 0 71) ;; 广东省自然科学基金团队项目 (粤科基办[2 0 0 3]11) ;; 广州市科技攻关项目 (2 0 0 3Z3-E0 4 0 1)
【CateGory Index】: TQ920
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【Citations】
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【Co-citations】
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1 ZHOU Shi-shui1, HU Fei2, JIANG Jian-guo2 (1.School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510640;2.College of Light Industry and Food Science,South China University of Technology,Guangzhou 510640);Optimization of fermentation conditions for secretively expressing HBscFv-IFNγ by engineering yeast X4[J];Industrial Microbiology;2008-04
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