Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of South China University of Technology(Natural Science)》 2005-01
Add to Favorite Get Latest Update

Expression and Modification of the Mutant of Human Acidic Fibroblast Growth Factor

Zheng Qing1 Su Zhi-jian1 Wu Xiao-ping2 Xu Hua2 Huang Zhi-feng1 Zhao Wen1 Yao Cheng-can2 Huang Ya-dong1 Li Xiao-kun2(1. Biopharmaceutical Research & Development Center, Jinan Univ., Guangzhou 510632, Guangdong, China; 2. Key Laboratory of Biopharmaceutics of Guangdong Province, Jinan Univ., Guangzhou 510632, Guangdong, China)  
The codons of the 98th and the 132nd cysteine (Cys) of haFGF (Human Acidic Fibro blast Growth Factor) cDNA were replaced with the serine (Ser) codon by means of PCR. Thus, the recombinant plasmid pET3c-haFGF Ser98,132 was constr ucted,which was then transformed into E. coli BL21 (DE3), with an expressio n level of 23.48%. The activity of the product was detected by MTT method, with the conclusion that the mutant of haFGF Ser98,132 is of an activity s imilar to standard haFGF. Maleimide-6-aminocaproyl-mPEG5000 ester was site-d irected couple to the 31st cysteine residue of haFGF Ser98,132. The result indicates that the modification rate is up to 30%, and the remained specific act ivity of the purified PEG-haFGF Ser98,132 is 55.53%.
【Fund】: 广东省自然科学基金资助项目 (0 10 4 2 4 ) ;86 3 计划资助项目 (2 0 0 2AA2Z3318)
【CateGory Index】: R346
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 YAO Cheng-can,ZHENG Qing,XU Hua,ZHAO Wen,WU Xiao-ping,SU Zhi-jian,HUANG Ya-dong,LI Xiao-kun(College of Pharmacy,Jinan University,Guangzhou 510632,China);The stability of recombinant human acid fibroblast growth factor[J];Chinese Journal of Modern Applied Pharmacy;2006-05
【Citations】
Chinese Journal Full-text Database 6 Hits
1 Li Xiao_kun, Wu Xiao_ping, Zheng Qing, Huang Ya_dong, Xu Hua, Yao Cheng_can, Zhao Wen (Biopharmaceutical Research & Development Center,Jinan University,Guangzhou 510632,China);Construction of High-Level Expression Strain of Human Acidic Fibroblast Growth Factor[J];Journal of South China University of Technology(Natural Science);2001-12
2 Lu Jun Liu Chunyu Liu Xiaoqin Feng Xiao Lin Jian (Bioengineering Research Institute,Jinan University,510632,Guangzhou);A Study on Fermentation Conditions of Recombinant Human bFGF in Eschierichia Coli[J];JOURNAL OF JINAN UNIVERSITY(NATURAL SCIENCE & MEDICINE EDITION);1997-03
3 HE Xianhui,NIE Huiling ** ,SHAW Pangchui 1) ,KE Yibao,TAM Siucheung 2) (Shanghai Institute of Cell Biology,Academia Sinica,Shanghai 200031;Departments of 1) Biochemistry and 2) Physiology, The Chinese University of Hong Kong,H;Site-Directed Polyethylene Glycol Modification of Trichosanthin[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;1999-06
4 YU Ying, CAI Shao Xi, XIA Yu Xian, Weririch HG (Institute of Bioengineering, Chongqing University, Chongqing 400044);Progress in the study on structure and functions of acidic fibroblast growth factor[J];Chinese Pharmacological Bulletin;2002-02
5 TIAN Hong, YAO Wen Bing, WU Wu Tong, SHENG Zi Long Department of Biochemistry, China Pharmaceutical University, Nanjing 210009;Chemical Modification of Activated Polyethylene Glycol to Recombined Human Interferon α-2b[J];JOURNAL OF CHINA PHARMACEUTICAL UNIVERSITY;2000-01
6 LI Xiao Kun, XU Hua, FU Xiao Bing, ZHENG Qing,HUANG Ya Dong, ZHAO Wen, WU Xiao Ping Biopharmaceutical Research and Development Center of Jinan University, Guangzhou 510632, China;Study of Recombinant Human Acidic Fibroblast Growth Factor on Improving Wound Healing in Rabbits[J];Journal of China Pharmaceutical University;2002-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Chun-li,FAN Xiu-jun,ZHAO Bing,YU Yang,LI Qing-zhang,HAO Yan-hong( Section of Physiology and Biochemistry of Animal Medicine Science College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC );Effect of induced concentration of IPTG on the expression of recombinant human augmenter ofliver regeneration gene[J];Journal of Northeast Agricultural University;2004-04
2 Guo Qin li, Wang Xiaofeng,Meng Juan, et al. Pharmacy College of Jinan University, Guangzhou 510630;The mitogenic activity of the non-mitogenetic human acidic fibroblast growth factor on nasopharyngeal and angioendothelial cells[J];Guangdong Medical Journal;2005-06
3 WU Zhi-ling, SU Zhi-jian, LI Xiao-kun, et al. Jinan University College of Pharmacy,Guangzhou 510632 China;Nonmitogenic acidic fibroblast growth factor inhibits the apoptosis of Balb/c 3T3 fibroblasts Induced by ActD[J];Guangdong Medical Journal;2006-02
4 Hu Shifeng,Huang Juen,Li Xiaokun, et al.Laboratory of Experimental Physiological Science, Guangxi Medical University, Nanning 530021 China;Protective effects of non-mitogen acidic fibroblast growth factor on injuries of carotid sinus[J];Anatomy Research;2003-03
5 WEI Jie1,2,YI Zhong2,WEI Yu-rong2,WANG Hai-feng1,2,HUERMAXI2,FU Zi-hua2,RAN Duo-liang1(1.Animal Medical College of Xinjiang Agriculture University,Urumqi 830052,China;2.Research Institute of Veterinary Science of Xinjiang Academy of Animal Sciences,Urumqi 830000,China);Effect of Induced Concentration and Time of IPTG on the Expression of VP1 Protein of FMDV Type Asia Ⅰ[J];China Animal Husbandry & Veterinary Medicine;2009-05
6 Wang Xiaoyuan Gong Wenqi Zhu Chunshui (Wuhan University of Technology Wuhan 430070);Application of Chemical Modification in Biosorption[J];Industrial Safety and Dust Control;2004-05
7 Li Xiao_kun, Wu Xiao_ping, Zheng Qing, Huang Ya_dong, Xu Hua, Yao Cheng_can, Zhao Wen (Biopharmaceutical Research & Development Center,Jinan University,Guangzhou 510632,China);Construction of High-Level Expression Strain of Human Acidic Fibroblast Growth Factor[J];Journal of South China University of Technology(Natural Science);2001-12
8 SHU Wei~1,HE Li-ping~1,CUI Tang-bing~2,GUO Yong~2(1 Test Center,South China Agric.Univ.,Guangzhou 510642,China;2 College of Biological Sciences and Engineering,South China Univ.of Tech.,Guangzhou 510640,China);Effects of Modification with 2-(O-Methoxypolyethyleneglycol)-4,6-Dichloro-s-Triazine on the Main Enzymological and Pharmacobiological Properties of Chymopapain[J];Journal of South China Agricultural University;2006-01
9 HUANG Tao~(1),HUANG Kai-xun~(2)(1.Key Laboratory for Analytical Chemistry of the State Ethnic Affairs Commission,College of Chemistry and Material Science,South-Central University for Nationalities,Wuhan 430074,Hubei,China;2.Department of Chemistry,Huazhong University of Science and Technology,Wuhan 430073,Hubei,China);Preparation and MALDI-TOF-MS Analysis of Acylated Insulin with Oleic Acid[J];Chemical Researches;2005-03
10 HUANG Tao~(1),HUANG Kai-xun~(2)(1.Key Laboratory for Analytical Chemistry of the State Ethnic Affairs Commission,College of Chemistry and Material Science,South-Central University for Nationalities,Wuhan 430074,China;2.Department of Chemistry,Huazhong University of Science and Technology,Wuhan 430073,China);Preparation and MALDI-TOF-MS analysis of the acylated insulin with linoleic acid[J];Chemical Research and Application;2006-07
China Proceedings of conference Full-text Database 1 Hits
1 WANG Hui-fang, LENG Wei-chun, ZHANG Su-ge, LIU Lan-fen, Gao Feng, YIN Wei-tian (PLA General Hospital, Jinan Military Area Command, Jinan 250031, China; Jilin University, Changchun 130062, China);Study on Gene Transfer and Biological Effects of aFGF in vivo[A];[C];2006
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Li Xiaokun,Fu Xiaobing *,Lin Jian(Bio-Engineering Institute of Jinan University,Guangzhou 510630);STUDY OF DOSAGE-EFFECT RELATIONSHIP BETWEEN THE WOUND-REPAIRING OF PIGS AND BASIC FIBROBLAST GROWTH FACTOR[J];JOURNAL OF GUANGXI MEDICAL UNIVERSITY;1999-02
2 Li Xiao_kun, Wu Xiao_ping, Zheng Qing, Huang Ya_dong, Xu Hua, Yao Cheng_can, Zhao Wen (Biopharmaceutical Research & Development Center,Jinan University,Guangzhou 510632,China);Construction of High-Level Expression Strain of Human Acidic Fibroblast Growth Factor[J];Journal of South China University of Technology(Natural Science);2001-12
3 Li Xiaokun Fu Xiaobing Hong An Feng Chengli Lin jian (Bio Engineering Institute, Jinan University Guangzhou 510632);STUDY OF RECOMBINATIVE BASIC RIBROBLAST GROWTH FACTOR FOR PHARMACODYNAMICS[J];WEST CHINA JOURNAL OF PHARMACEUTCAL SCIENCES;1999-Z1
4 LI Xiao-kun 1, HONG An 2, XU Hua 1, YAO Cheng-can 1, FU Xiao-bing 3, LIN Jian 2 (1. Biopharmaceutical R&D Center, Jinan University, Guangzhou 510632, China; 2. Bioengineering Research Institute, Jinan University, Guangzhou 510632, China; 3. T;The clinical study of recombinant bovine basic fibroblast growth factor on wounds healing[J];Journal of Jinan University(Natural Science & Medicine Edition);2002-02
5 XU Hua,ZHENG Qing,YAO Cheng-can,YANG Xiao-ming,WANG Yan-ping,LI Xiao-kun(Biopharmaceutical Research & Development Center, Pharmacy College, Jinan University, Guangzhou 510632, China) ;Experiment study of recombinant human acidic fibroblast growth factor on improving scald wound healing in rats[J];Journal of Jinan University(Natural Science & Medicine Edition);2003-02
6 Luo Min; Cao Wenjun; Chen Shujing; Ji Qiuhe; Zhao Yu; Chen Jialun (Shanghai Institute of Endocrinology, Shanghai 200025);Immunoenzymometric Assay ELISA for Human Thyrotropin[J];SHANGHAI J.OF IMMUNOLOGY;1996-03
7 RENFengqin ,SONGYaohong , FENGTao (ChineseAcademyofMedical SciencesandPekingUnionMedicalCollege, PekingUnionMedicalCollegeHospital, Beijing1 0 0 730 ,China) .;Studieson the Optimal Conditions for the Determination of Microa lbuminin Urine by ELISA .[J];Progress In Biochemistry and Biophysics;1997-01
8 Gao Ningguo, Cheng Xiulan, Yang Jing and Zhang Shuzheng( Institute of Microbiology, Chinese Academ y of Sciences, Beijing 100080);Progress in Heparin Structure and Function[J];PROGRESS IN BIOTECHNOLOGY;1999-05
9 GUO Lijun, LI Xiaokun.XU Hua et al( Changchun Institute of Biological Products, Changchun 130062);Curative Effect of Recombinant Bovine Basic Fibroblast Growth Factor on Burn Wound Degree II[J];Chinese Journal of Biologicals;2002-05
10 ZHAO Wen-xing, ZHAO Jing,LIANG Chong-li(Kunming General Hospital ,Chengdu Command, Kunming 650032,China);Influence of polyphenols on MTT method in detecting cell activity[J];The Chinese Journal of Modern Applied Pharmacy;2004-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Li Xiaokun Fu Xiaobing Hong An Feng Chengli Lin jian (Bio Engineering Institute, Jinan University Guangzhou 510632);STUDY OF RECOMBINATIVE BASIC RIBROBLAST GROWTH FACTOR FOR PHARMACODYNAMICS[J];WEST CHINA JOURNAL OF PHARMACEUTCAL SCIENCES;1999-Z1
2 Wang Shuqi and Cheng Yuhua (Department of Chemistry, Jilin University, Changchun);THE IMPROVEMENT OF STABILITY OF ELASTASE BY CHEMICAL MODIFICATION[J];Journal of Jilin University;1985-02
3 Zhou Li;Luo Guimin;Gao Shujuan; Sun Qian; Qi Danhua (Laboratoly of Enzyme Engineering, Jilin University, Changchun, 130023)Guo Zhongman;Sun Changrong;Gou Hongying;Hu Ying;Wang Yang (Pharmaceutical Factory of Triumph, Changchun, 130052);Modification of Bovine Serum Albumin with Polyethylene Glycol[J];ACTA SCIENTIARIUM NATURALIUM UNIVERSITATIS JILINENSIS;1997-04
4 Lu Jun Liu Chunyu Liu Xiaoqin Feng Xiao Lin Jian (Bioengineering Research Institute,Jinan University,510632,Guangzhou);A Study on Fermentation Conditions of Recombinant Human bFGF in Eschierichia Coli[J];JOURNAL OF JINAN UNIVERSITY(NATURAL SCIENCE & MEDICINE EDITION);1997-03
5 Zheng Baosheng;Xu Mingbo;Shen Beifen(Institute of Basic Medical Sciences, Academy of Military Medical Sciences);Chemical modification of recombinant human interleukin-2[J];BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES;1994-01
6 Guan Yingjun,Gao Yingmao ,Juan Zhaodong,et al Department of Histology and Embryology,Weifang 261042 Shandong Medical University;EFFECTS OF aFGF ON THE DIFFERENTIATION AND ITS RECEPTOR EXPRESSION OF NEUROEPITHELIAL CELLS IN PRIMARY CULTURE[J];JOURNAL OF WEIFANG MEDICAL COLLEGE;1999-01
7 GAO Jian xin, XIN Hua,ZHANG Heng,et al(Dept.of Physiology,Shandong Medical University);THE EFFECTS OF NGF AND aFGF ON THE GROWTH AND DEVELOPMENT OF EMBRYONIC SPINAL CORD NEURONS IN VITRO[J];ACTA ACADEMIAE MEDICINAE SHANDONG;2000-03
8 LONG Jianyin, WANG Huixin ( Beijing Institute of Basic Medical Sciences , Beijing 100850, China).;Advances in the Study of Foreign Gene Expression in E.coli.[J];Progress In Biochemistry and Biophysics;1997-02
9 HE Xian-Hui;KE Yi-Bao;CAI Ying-Chun and NIE Hui-Ling(Shanghai Institute of Cell Biology,Academia Sinica,200031);Effects of Chemical Modification of Lysine and Arginine Residues of Trichosanthin on its Reactivity with IgE[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1994-06
10 YUAN HuiDong, LI MoYi, SHAO XiaoXia, CUI DaFu, XIA QiChang and ZHANG ZuChuan( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China );Determination of the Binding Epitope for Antitrichosanthin Monoclonal Antibody T8C12[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1998-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved