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《Journal of South China University of Technology(Natural Science Edition)》 2008-04
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Separation of Active Components from Andrographis paniculata Using High-Speed Counter-Current Chromatography

Zhang Yun-feng1 Wei Dong2 Guo Si-yuan2 Chen Feng2(1.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China;2.Research Institute of Light Industry and Chemical Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China)  
In order to efficiently separate high-purity active components from Andrographis paniculata,a high-speed counter-current chromatography(HSCCC) procedure was developed for the preparative separation and purification of four bioactive compounds from the crude extract of the plant in a simultaneous process.The procedure was performed in a two-phase solvent system of n-hexane-ethyl acetate-ethanol-water(in a volume ratio of 1∶4∶2.3∶2.7) at a flowrate of 2.0mL/min.By determining the physicochemical properties and according to the results of MS,1HNMR and 13CNMR,the four separated compounds were identified as 14-deoxy-andrographolide-19-β-Dglucosides(Ⅰ),neoandrographolide(Ⅱ),andrographolide(Ⅲ) and 14-deoxy-andrographolide(Ⅳ).Moreover,56.8,1.5,108.3 and 218.0 mg of compounds Ⅰ~Ⅳ were respectively prepared from 500mg of crude extract by the proposed procedure,with the purities of 98.5%,92.5%,96.2% and 93.6% and the recoveries of 95.5%,97.8%,93.2% and 91.9%,respectively.
【Fund】: 广东省自然科学基金资助项目(05006524)
【CateGory Index】: R284
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