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《农业科学与技术(英文版)》 2017-05
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Study on the Phenotypic Mutation Generated from ~(60)Coγ-ray Irradiated Oxlias triangularis Purpurea Regeneration System

Jie REN;Qi ZHU;Yanqiong MENG;Fei TANG;Yun XUAN;Dan SHI;Jingjing WANG;Yongjin DENG;Songling FU;Institutes of Agricultural Engineering,Anhui Academy of Agricultural Sciences;College of Forestry and Landscape Architecture,Anhui Agricultural University;  
Mutations induced from tissue culture are easily to be separated,which might be propagated in the same medium,especially for the color-leaf ornamental grass,Oxalis triaggularis purpurea.Mutations of the ornamental traits in the tissue culture bottle could be investigated easily.The 35 d regeneration system group showed the lowest adventitious bud number and adventitious root number among 4 inoculation dates by 50 Gy dose of ~(60)Coγ radiation.More studies were carried out based on the 35d differentiation state of O.triangularis purpurea regeneration system.The 35 d regeneration system was then irradiated by 10,25 and 50 Gy doses of ~(60)Coγ rays.Numbers of adventitious buds and roots induced from the regeneration system were cut down with the increment of radiation doses.Seedling length was not distinctly reduced at the absorbed doses of 10 and 25 Gy,but reduced distinctly under 50 Gy of ~(60)Coγ irradiation.The optimal irradiation dose for 35 d O.triangularis regeneration system survival and mutation induction was approximately 25 Gy.The M_2 phenotypic mutation rate was 2.9%,especially,and the leaf number mutation accounted for 76%of the total mutation.The phenotypic mutations,especially in the 10 Gy group,on 0.1 m M Vc containing MS medium were recovered,which indicated that ROS plays a key role in the phenotypic mutation induced by ~(60)Coγ -rays.
【Fund】: Supported by the National Natural Science Foundation of China(31600543);; Anhui Provincial Natural Science Foundation(1708085MC57);; the State Forestry Administration China(“948”Project:#2014-4-52)
【CateGory Index】: S68
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