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《农业科学与技术(英文版)》 2017-05
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Physiological Responses of Actinidia arguta (Seib.et.Zucc.) to Low Temperature Stress

Li MA;Tingsong GUO;Xuesen CHEN;Dekui ZANG;Xiaojun GONG;Jingjing SUN;Yanfen QIU;Yanling WANG;College of Forestry,Shandong Agricultural University;Culai Forest Farm of Tai'an;  
[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta(Seib.et.Zucc.), and provide a theoretical basis for the introduction and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chlorophyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes(POD activity, SOD activity, CAT activity) with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time,the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually,while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend,meanwhile the activities of CAT was first increased and then decreased. [Conclusion] The various indicators showed that under low temperature stress, the chlorophyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant enzymes play a protective role in different degrees.
【Fund】: Supported by the Special Fund for Agro-scientific Research in the Public Interest (201303093);; the Key Project for Agricultural Improved Variety Breeding of Shandong Province (Lunongliang[2016]No.6~~
【CateGory Index】: S663.4
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