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《农业科学与技术(英文版)》 2017-05
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Optimization of IBRS-2 Cells Culturing Conditions for Cultivation of Porcine Parvovirus N Strain Using Roller Bottle

Bingxia LU;Ying HE;Wu ZHAO;Jiaxing LIANG;Qunpeng DUAN;Dongfu JIANG;Jingzhuan LU;Bingfen BI;Yingning ZHOU;Yibin QIN;Bin LI;Qianlian SU;Zhongwei CHEN;Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Veterinary Biotechnology;  
The aim was to optimize the roller bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus(PPV) N strain using a four-factor three-level orthogonal test(cell culture medium p H value: 7.0, 7.2 and 7.4; inoculation time: 0,24 and 48 h; inoculation dose: 0.10%, 1.00% and 10.00%; harvest time: 48, 72 and96 h). The results showed that the optimal conditions were as follows: cell culture medium p H value of 7.2, synchronous inoculation, inoculation dose of 0.10% and culture time of 96 h. Among the four factors, culture time was the most important factor affecting the virulence titer of PPV N strain.
【Fund】: Supported by Nonprofit Institute Research Grant of Guangxi Province(GK15-2 GK16-2);; Guangxi Aquatic Animal Husbandry Science and Technology Project(GYMK201528030 201633034)~~
【CateGory Index】: S852.651
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