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《Journal of Henan Agricultural Sciences》 2007-01
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Important Factors Affecting Wheat Tissue Culture Efficiency

ZHAO Yong-ying~1;LI Cui-xiang~2;MIAO Hong-mei~(1*)(1.Henan Key Laboratory for New Crop Improvement,Zhengzhou 450002,China;2.Grain College,Henan University of Technology,Zhengzhou 450052,China)  
In order to improve the efficiency of wheat tissue culture,the effects of 2,4-D,AgNO_3 and sucrose on callus induction and regeneration were studied by culturing the scutella from Zhengmai 9023.The results showed that the medium with 0.5 mg/L 2,4-D was better for the inducement of embryogenic callus;additional 30 g/L sucrose was beneficial to wheat green primordium differentiation,while a supplement of 5 mg/L or 10 mg/L AgNO_3 was negative.
【Fund】: 河南省杰出人才创新基金项目(0121000700);; 河南省自然科学基金项目(0611032100)
【CateGory Index】: S512.1
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