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《Journal of Henan Agricultural Sciences》 2007-01
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The fad2 Gene Transformation of Brassica napus Mediated by Agrobacterium

LIANG Hui-juan~1,CAO Gang-qiang~1,MIAO Li-juan~2,TIAN Bao-ming~1,WENG Hai-bo~1,HUANG Bing-yan~2,CHEN Zhan-kuan~(2*)(1.Department of Biology Engineering,Zhengzhou University,Zhengzhou 450001,China;2.Biotechnology Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)  
Delta-12 oleate desaturase gene(fad2)is a rate-limiting gene for multi-unsaturated fatty acid synthesis in rapeseed(Brassica napus).Blocking the expression of fad2 gene could increase the comparative content of the linolic acid in rapeseed.The authors constructed the RNAi expression vector-pCAMBIA3301-fad2i targeting the gene fad2 by RNAi strategy.Using the cotyledons with petiole of rapeseed cultivar Y141 as material,the gene fad2 RNAi constructs was introduced into rapeseed by.A.tumefaciens-mediated method.Some PPT-resistant green shoots were obtained.At the same time,some factors which affected the transformation of cotyledons with petiole were studied.The results of GUS assay and PCR amplification indicated that the foreign gene was stably integrated and expressed in the genome of rapeseed.
【CateGory Index】: S565.4
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