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《Journal of Henan Agricultural Sciences》 2007-01
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Multiplex PCR for Detection of Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus 2(PCV-2)

ZHOU Hai-fan,XIA Ping-an~*,CUI Bao-an,ZHANG Hong-ying,HU Mei,DANG Zhan-guo(College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China)  
Based on conserved sequences in ORF6 and ORF7 fragments of PRRSV American strain(ATCC VR-2332) and that in ORF2 of PCV-2(DQ195679)strain,two pairs of specific primers were respectively designed,through optimizing of RTPCR and PCR conditions,multiplex PCR for detection of PRRSV and PCV-2 was established.Fifteen organ samples from different farms of Henan province were respectively detected by the duplex PCR,out of which eight were PRRSV positive,five were PCV-2 positive,three were both PRRSV and PCV-2 positive,the control samples were negative,suggesting that the multiplex PCR of PRRSV and PCV-2 was a rapid, specific and sensitive diagnostic method.
【CateGory Index】: S854.43;S858.28
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