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《Journal of Henan Agricultural Sciences》 2007-03
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Identification and Analysis of the SRAP Markers Linked with Powdery Mildew Resistance Gene in Wheat

YI Yan-jie1,2,HU Nan3,LIU Hong-yan2,AN Li-zhe1,LIU Xin-tao2,WANG Xun-ling1 (1.Key Laboratory of Arid and Grassland Ecology of Ministry of Education,Lanzhou University,Lanzhou 730000,China;2.Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;3 Nanyang Institute of Technology,Nanyang 473004,China)  
240 SRAP primer combinations were applied to amplify wheat cultivar SF42(highly susceptible to powdery mildew) and ZB90(immune to powdery mildew).Forty percent of primer combinations could amplify the stable polymorphic bands.Further analyzing the F2 segregating populations from the cross of SF42 and ZB90 using these primers,there are five SRAP markers produced by four primer combinations Me5+Em5、Me8+Em7、Me8+Em16 and Me12+Em7,which were linked with the powdery mildew resistance gene in ZB90.These markers were recovered,cloned and sequenced.Sequence analysis showed these markers contained ORFs and four of ones had conserved motifs such as signal-peptide and transmembrane-regions.
【Fund】: 河南省杰出青年科学基金项目(04120001400);;
【CateGory Index】: S512.1
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Shuang-shuang,ZHAO Rui-hua,YAN Gui-qin,HAO Gang(Institute of Biodiversity,School of Life Science,Shanxi Normal University,Linfen 041004,Shanxi,China);Establishment & Optimization of SRAP Analysis System of Potato Genome[J];Journal of Shanxi Normal University(Natural Science Edition);2007-04
2 Ren Yu1 Zhang Yindong2 Yin Junmei1 Wang Deyuan3 1 Tropical Crops Genetic Resources Institute,CATAS Danzhou Hainan 571737 2 State Key Laboratory for Biotechnology of Tropical Crops,CATAS Haikou 571101 3 Vegetable Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Biotechnology Laboratory for Fruits and Vegetables Guangzhou 510640;SRAP Assessment of Genetic Relationship of Inbred Hot Pepper (Capsicum annuum L)[J];Chinese Journal of Tropical Crops;2008-01
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4 DENG Si-li1,PAN Jun-song1,HE Huan-le1,WU Ai-zhong1,2,CAI Run1 (1.School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 201101; 2.Shanghai Academy of Agricultural Sciences, Shanghai 201106, China);SRAP Molecular Marker Linked to M Gene in Cucumber[J];Journal of Shanghai Jiaotong University(Agricultural Science);2006-03
5 MA Ming1,YANG Ke-qiang1,LIU Xiao-jü1,SUN Ming-gao1,GUO Qi-rong2(College of Forestry,Shandong Agriculture University,Taian 271018 China;2.International Center for Bamboo and Rattan,100102,P.R.China);ESTABLISHMENT OF SRAP MARKER REACTION SYSTEM IN WALNUT[J];Journal of Shandong Agricultural University(Natural Science Edition);2007-02
6 ZHANG Zhi-wei~1,HAN Yao-ping~1,CAO Zhe-ming~2(1.Wuxi Fishery College,Nanjing Agricultural University,Wuxi 214081,China;2.Freshwater Fishery Research Center,Chinese Academy of Fishery Science,Wuxi 214081,China);Establishment of TRAP-PCR Reaction Conditions in Grass Carp[J];Biotechnology;2006-02
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