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《Journal of Henan Agricultural Sciences》 2007-03
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Expression of Protein Encoded by λ Light Chain Gene of Bursal B Lymphocytes in E.coli

SONG Hai-tao1,2,ZHANG Hong1,2,3,WANG Li2,DUAN Yan-hua1,2,QIAO Song-lin1,2,LI Xue-wu2,ZHAO Guang-hui1,2,ZHANG Gai-ping2* (1.Henan Agricultural University,Zhengzhou 450002,China;2.Henan Key Laboratory of Animal Immunology,Zhengzhou 450002,China;3.Henan Institute of Education,Zhengzhou 450003,China)  
λ light chain gene encoding the expression protein was amplified by polymerase chain reaction(PCR) from infectious bursal B lymphocyte cDNA library, and inserted into the corresponding sites of vector pUC19 after digested by restriction endonuclease to construct protocaryon expression vector PET43a.Both nucleotide sequencing and BamHⅠ and HindⅢ digestion confirmed that λ light chain gene fragment encoding the target protein was correctly inserted into the vector.The recombinant plasmid was transformed into E.coli BL21(DE3) and induction by IPTG and SDS-PAGE indicated that the target protein was expressed.
【Fund】: 国家自然科学基金重点项目(30203270)
【CateGory Index】: Q786;S852.65
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Hong1,2,3,ZHANG Gai-ping1,ZHANG Jin-gang2,YANG Yan-yan1,YI Ming-lin1,LI Jiao1,HAN Hong-peng31Academy of Agricultural Sciences in Henan,Zhengzhou 450002;2Academy of Military Medical Sciences,Beijing 100850;3Henan Educational Institute,Zhengzhou 450003,China;Secretable eukaryotic expression of recombinant chicken Igλ and preparation of monoclonal antibodies against chicken Igλ[J];Chinese Journal of Cellular and Molecular Immunology;2008-03
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