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《Journal of Henan Agricultural Sciences》 2007-11
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Construction of Rapeseed CBF1 Plant Expression Vector and Its Transformation to Arabidopsis thaliana

DUAN Hong-ying,DING Xiao-sheng (The College of Life Science,Henan Normal University,Xinxiang 453007,China)  
In this study,CBF1 gene was cloned from rapeseed by RT-PCR,then was constructed into plant expression vector pCAMBIA1304.The recombinant expression vector was transformed into Arabidopsis thaliana by means of Floral dip with Agrobacterium tumefaciens.Hygromycin screening and PCR assay confirmed that the rapeseed CBF1 gene was successfully transformed into Arabidopsis thaliana.
【Fund】: 河南省新乡市科技攻关项目(06N059);; 河南师范大学基金项目(2006011 06114)
【CateGory Index】: S565.4
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
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4 LI Yan,SHEN Shu-xing,XUAN Shu-xin,LI Xiao-feng,WANG Yan-hua(College of Horticulture,Agricultural University of Hebei,Baoding 071001,China);Cloning the cDNA of DREB-like Transcription Factor from Chinese Cabbage and Construction of Plant Expression Vector[J];Acta Agriculturae Boreali-Sinica;2009-01
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6 Zhu DongmeiJia YuanCui JizheFu Chang(College of Life Sciences and Biotechnology,Harbin Normal University,Harbin 150025 );Plant Transcription Factors in Response to Salt Stress and Its Biological Characteristics[J];Biotechnology Bulletin;2010-04
7 YAO Wu-Lan,ZHANG Zeng-Yan*,CHEN Liang,and XIN Zhi-Yong(National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Genetic and Breeding/Ministry of Agriculture/Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Cloning and Expression of a Pathogen-induced ERF Gene in Thinopyrum intermedium[J];Acta Agronomica Sinica;2007-09
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