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《Journal of Henan Agricultural Sciences》 2007-11
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Isolation and Inhibitory Effects of Phyllosphere Actinomycetes against Botrytis cinerea on Cucumber

WANG Zhen-yue,LIU Yan-hong,GAO Shu-feng,YUAN Hong-xia,LI Hong-lian (College of Plant Protection,Henan Agricultural University,Zhengzhou 450002, China)  
Forty-five strains of phyllosphere actinomycetes were isolated from phyllosphere of cucumber in the fields and greenhouses at suburb of Zhengzhou with methods of "plating of leaf washings" and "plating of diluted leachates from sterilized,grinded leaves".The results of dual culture on PDA plate in vitro showed that 6 strains of actinomycetes,including S32 and S10,were capable of strong antagonism against Botrytis cinerea Pers.The culture filtrate of S32,S10 and S23 had obvious inhibitory effects on the mycelial growth of Botrytis cinerea Pers.The inhibitory effect of the diluted culture filtrate of S23 with 10 times dilution was better than that of 50% Procymidone of 1000 times dilution.
【Fund】: 河南省教育厅科技攻关项目(2000210028)
【CateGory Index】: S436.421.1
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