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《Journal of Henan Agricultural Sciences》 2007-11
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Studies on the Transformation Parameters of Leaf Disc of Chrysanthemum in Virtue of Transient Expression of Gus Gene by Particle Bombardment

SUN Lei,ZHANG Qi-xiang (National Flower Engineering Technology Research Center,College of Landscape Architecture, Beijing Forestry University,Beijing 100083,China)  
In order to apply particle bombardment to the genetic improvement and variety breeding of chrysanthemum,gus gene was used as report gene, then transformed into the explants of chrysanthemum by particle bombardment.The transient expression of gus gene in the leaf discs of chrysanthemum were examined to analyze the effects of shoot distance,helium pressure,gold particle amount,plasmid DNA concentration,culture-resuming time and bombarding frequency on the expression of gus gene.The results showed that,in the conditions of 6 cm shoot distance,7.584×106Pa helium pressure,400μg gold particle per shoot,3μg plasmid DNA and bombarding for 2 times,the most active transient expression of gus gene could be detected two days after particle bombardment.The transient expression of gus gene was also effected by bombarding frequency.
【Fund】: 教育部博士点基金项目(20040022022);; 科技部国家转基因专项项目(JY03-B-28)
【CateGory Index】: S682.11
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【Co-citations】
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