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《Journal of Henan Agricultural Sciences》 2007-11
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Molecular Cloning and Antigenicity Analysis of VP2 Gene from PPV HN-3 Strain

LIANG Xiu-li1,FANG Li-yun2,WANG Dong-fang2,MO Peng-tao2,FU Yun-fei2,WEI Zhan-yong2 (1.Anyang University of Technology,Anyang 455000,China;2.College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China)  
A pair of primers was designed according to the sequence of NADL-2 strain from GenBank,and was used to amplify the major antigen regions of VP2 of HN-3 strain by PCR.The PCR products were then cloned into pGEM-T Easy vector.A 1438bp nucleotide fragment was acquired,with a deduced peptide of 475 amino acids.Sequence comparison showed that the nuc-leotide and amino acid sequences of VP2 of HN-3 strain were 99.4% and 99.3% similarity,respectively,with those of NADL-2 strain.The VP2 antigen epitopes were predicted using DNA-Star software,indicating that eleven antigen epitopes were located,respectively,at the N-terminal region of 11-24,81-108,121-135,139-148,150-172,221-239,241-259,316-342,344-352,390-405 and 426-439.
【CateGory Index】: S852.65
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