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《Journal of Henan Agricultural Sciences》 2007-11
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Analysis and Application of E.ovis Antigen

ZHANG Shou-fa,JIA Li-jun (Agricultural College of Yanbian University,Longjing 133400)  
The antigen trait and immunogenicity of E.ovi were analyzed using SDS-PAGE and western blotting,then the E.ovis antigen was used as a diagnostic antigen to detect 64 serum samples by ELISA.The results showed that the 56kD and 26kD antigens had better immunogenicity and could not react with the sheep positive serum of Mycoplasma pneumonia and negative serum of E.ovis.Of the 64 samples detected,the positive rate was 66.7%(42/64),22.9% higher than that of direct microscopic examinerion.
【CateGory Index】： S858.27
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