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AFLP and Specific Fragment Sequence Analysis of T5 Generation Rice Variants

JI Sheng-dong1,CHEN Peng1,JIAO Zhen2,ZHU Hong-yan1WANG Jia-chuan1,YUAN Zhao1,YUE Chun-hui1,WANG Zhi-feng1,QIN Guang-yong2(1.College of Life Science,Henan Normal University,Xinxiang 453007,China;2.Industrial Institute of Zhengzhou University,Zhengzhou 450052,China)  
The rice variety Xiang 1 was used as material to screen 64 pairs of PstⅠ-MseⅠ selective primers,from which 19 pairs was confirmed to produce the appropriate and clear amplified bands.Then the 19 pairs of primers were used to do AFLP analysis on four T5 generation variant lines with different phenotype characters and their two parents.The result showed that the genome of variants changed significantly in comparison to Xiang 1.The specific fragments p38-114 from variant 06-5-114-11-2 and p82-108 from variant 06-5-108-11-3 were cloned and sequenced,and their base similarities were compared in NCBI database,which indicated that p38-114 presented 99% sequence similarity with mRNA NM_001074855.1 and p82-108 had 100% sequence similarity with mRNA NM_001061652.1.
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