Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Decomposable Ability of F_1 Strain to Lignin and Cellulose of Corn Stalk

DUAN Ya-bing,CHEN Yang-yang,KANG Ye-bin*(Henan University of Science and Technology,Luoyang 471003,China)  
The present paper aimed to study the enzyme-producing activity of F1 strain and its degradation ability to lignin and cellulose of corn stalk.The culture medium Ⅰ was prepared by adding carboxymethylcellulose sodium as inducer,and medium Ⅱ without carboxymethylcellulose sodium as CK.Then the F1 strain was inoculated on the two media,respectively,and was induced to produce cellulose degeneration enzyme Cx at 25℃.The activity of Cx was determined by spectrophotometric method at different time.After 5-day culture,the F1 strain nutrient fluid was transferred to corn stalk solid fermentation medium with a vaccination amount of 10% and cultured for 20 days.At 5,10,15 and 20 days,the degradative ability of F1 strain to hemicellulose,cellulose and lignin of corn stalk was determined by dispersion method.The results showed that the F1 strain could produce Cx enzyme on culture medium with carboxymethylcellulose sodium,and its enzyme activity reached the highest in 5 days of culture.The F1 strain grew well in corn stalk fermentation medium,and maintained a high degradation activity to lignin and cellulose in the early 15 days.After that,the rates of degradation to cellulose decreased significantly and even stopped.After cultured for 20 days,the accumulative rates of degradation to hemicellulose,cellulose and lignin were 3.5%,10.5% and 7.0%.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved