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Effects of Bacillus thuringiensis as Food Additive on the Phagocytic Activity of Leucocytes and Lysozyme Activity of Cyprinus carpio in the Yellow River

YIN Hai-cheng(College of Bioengineering,Henan University of Technology,Zhengzhou 450001,China)  
To study the effects of Bacillus thuringiensis(Bt) on the leucocyte and lysozyme activities of Cyprinus carpio in the Yellow River,the fishes of group A1,A2 and A3 immunized with A.hydrophila and the unimmunized fishes of group B1,B2,and B3 were feed with the forage added with Bt at the concentrations of 1.0×1011,3.0×1011 and 5.0×1011 cfu/kg,respectively.The phagocytic activities of leucocytes and lysozyme activities were determined at 1、15、30 and 40 days post-feeding.The results showed that all the different concentrations of Bacillus thuringiensis enhanced the phagocytic activities of leukocytes and lysozyme activities of Cyprinus carpio in the Yellow River(P0.05),but high dose groups showed higher effects than that of low dose groups.No obvious different phagocytic activities of leukocytes were observed among the immunized and unimmunized groups(P0.05),but the lysozyme activities were significantly different(P0.05).The phagocytic activities of leucocytes and lysozyme activities declined at 10d after the stopping of feeding mixed diet,but no significant differences were observed compared to that of feeding mixed diet(P0.05).
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