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Effects of Cytochalasin B on the Efficiency of Somatic Cell Nuclear Transfer in Yanbian Yellow Cattle

SONG Jun-bo,LIN Tao,HU Yan-ming,LI Zhong-shu,FANG Nan-zhu*(College of Agriculture,Yanbian University,Longjing 133400,China)  
To investigate the best conditions of electrofusion and activation for nuclear transplantation in Yanbian yellow cattle,the cytochalasin B(CCB) was added in the medium of fusion and activation and the efficiency of somatic cell nuclear transfer was determined.The result showed that the morula rate and the rate of development into blastocyst stage of the group added with 7.5μg/mL CCB in fusion medium were significantly higher than that of other groups.The clea-vage rate,morula rate and the rate of development into blastocyst stage of the group added with 5.0 μg/mL CCB in activation medium were significantly higher than that of other groups.When the CCB was added in the fusion and activation media together,the rate of development into blastocyst stage was slightly higher than that added with CCB in fusion or activation medium alone,but no significant difference was observed.
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