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《Journal of Henan Agricultural Sciences》 2009-06
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Optimization of Seed Sterilization Conditions for Aseptic Seedling on Brassica napus L.

LI Xu-jiao1,TIAN Bao-ming1*,FAN Xing-fu1,SHI Gong-yao1,SONG Zheng2,PEI Zhen-qiang1,WANG Bo-nan1,SUN Dan-dan1,GAO Shu-guang1(1.Bioengineering Department,Zhengzhou University,Zhenzhou 450001,China;2.Henan Institute of Standards,Zhengzhou 450002,China)  
This experiment was conducted to find out the best sterilization method for the sterile rapeseed seedling through investigating effects of different disinfectant agents,storage duration and temperature on the rapeseed before cultured.The results showed that the aseptic seedling for transgenic could be obtained by the new rapeseed stored in a refrigerator(4℃),sterilized 0.5-1 min with 70% alcohol,sterilized 10 min with 0.1% HgCl2,washed 5-6 times with aseptic water and soaked in aseptic water 4-5h,and then cultured on MS under 25℃ at dark condition for 1-2d,then at 16 hours light condition for 4-5d.
【Fund】: 河南省重大攻关项目(072101110300);; 河南省自然科学基金(0511030600)
【CateGory Index】: S565.4
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