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Bioactivity and Identification of Bacillus spp. SH6-1

LI Ji-shun,CHEN Kai,HU Jin-dong,WANG Zhao-na,YANG He-tong*(Biotechnology Center of Shandong Academy of Sciences,Key Laboratory for Applied Microbiology ofShandong Province,Jinan 250014,China)  
Bacillus spp.isolate SH6-1 was isolated from the soil of cucumber in Shouguang city of Shandong province,based on physiological and biochemical characteristics and 16S rDNA sequencing results,isolate SH6-1 was identified as Bacillus megaterium.Strain SH6-1 was capable of fixing nitrogen,mobilizing insoluble phosphate and potassium simultaneously.The supernatant fluid of 5d culture had 3.573μg/mL of nitrogen,and 9.139μg/mL of KCl.The supernatant fluid of 2d culture had 2932.5μg/mL of P2O5.In greenhouse test,strain SH6-1 had increased 8.8% and 33.8% in wheat stem length and fresh weight.Strain SH6-1 had 12.2% and 15.4% increases in tomato stem length and fresh weight and 57.8% and 61.7% control efficiency against wheat sharp eyespot and tomato seedling damping-off.
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