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Effects of MnSO_4 on Mitosis of Garlic Root Tip

WANG Yi,QIU Feng-tong(College of Life Science,Linyi Normal University,Linyi 276005,China)  
The garlic root tips were treated with different concentrations solutions of MnSO4(1.0,2.0,3.0,4.0,5.0 mmol/L) lasting different time(12,24,48h),and then observed the mitosis with normal chromosome pressing technology.The results showed that with the increase of the concentration of MnSO4,the mitotic index presented an M-shape curve;with the lengthening of treatment time,the mitotic index decreased gradually,which explained that MnSO4 might be the mitotic inhibitor.Furthermore,the phenomena of chromosomal aberration such as chromosome breakage,chromosome bridge and micronucleus were also observed during mitosis.
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