Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Henan Agricultural Sciences》 2009-12
Add to Favorite Get Latest Update

Expression and Purification of Hog Cholera Lapinised Virus E~(rns) Gene in Escherichia coli

WANG Li1,YANG Yan-yan1,ZHANG Hong2,LI Qing-zhou3,LI Xue-wu1,ZHANG Gai-ping1(1.Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.Henan Educational Institute,Zhengzhou 450003,China;3.Agricultural Economicsand Information Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)  
The full lengh of Erns gene was subcloned into the prokaryotic expression vector pGEX-6p-1.The results of PCR amplification,restriction enzyme digestion and DNA sequencing showed that the recombinant plasmid was obtained.Then it was transformed into E.coli BL21(DE3)competent cells for expression.The expressed protein was identified by SDS-PAGE and Western-Blot,which showed the molecular weight of Erns fusion protein was approximately 55KD.E.coli BL21(DE3) harboring the recombinant plasmid pGEX-6p-1-Erns expresses the protein at high level(45%).The expressed protein was specifically recognized by rabbit anti-CSFV polyclonal antibodies.Ernsprotein was purified by GST-chelating chromatography.This may provide tools for further development of diagnostic reagent for detecting CSF antibodies.
【Fund】: 国家“863”计划项目(2007AA100606)
【CateGory Index】: S852.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 ZHANG Gai-ping1,LI Qing-zhou1,2,HAO Hui-fang1,ZHOU Ling1,WANG Li1,YANG Su-zhen1,LUO Jun1,FENG Li-li2 (1.Henan Key Laboratory for Animal Immunology,Zhengzhou 450002,China;2.Engineering College of Animal and Vetrinary Science,Henan Agricultural University,Zhengzhou 450002,China);Expression of NA Gene of Avian Influenza Virus Subtype H5N1 in Escherichia coli and Purification of the Expressed Product[J];Acta Agriculturae Boreali-Sinica;2008-05
2 XU Bao-jiang1,2,CHEN Lu1,YOU Yi1,2,LIU Chun-sheng1,ZHENG Lu-ping1,WANG Chuan-qing1(1.College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China;2.Institute of Farming and Veterinary,Henan Academy of Agriculture Sciences,Zhengzhou 450002,China);Preparation of Monoclonal Antibodies against Classical Swine Fever Virus and Their Identification of Biological Characters[J];Acta Agriculturae Boreali-Sinica;2009-03
3 ZANG Jin-can,FAN Guo-yan,QIAO Hong-xing,LIU Wei-min(Zhengzhou College of Animal Husbandry Engineering,Zhengzhou 450011,China);Comparative Research on Detecting Swine Fever Virus by RT-PCR and Rabbit-cross Test[J];Journal of Henan Agricultural Sciences;2007-01
4 LI Xue-wu1,ZHANG Gai-ping1,GUO Qi-xiang2(1.Henan Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 2.Henan Entry-exit Inspection and Quarantine Bureau,Zhengzhou 450003,China);Studies on Biological Characteristics of Classical Swine Fever Virus[J];Journal of Henan Agricultural Sciences;2007-10
5 LI Ya-li1,2,3,ZHOU Jie1,2,LI Jing-xi1,2,LI Qiao-mu3,4,WANG Li3,CHAI Shu-jun3,WANG Xuan-nian1,2,3*(1.Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China;2.Henan Institute of Science and Technology,Xinxiang 453003,China;3.Henan Provincial Key Laboratory of Animal Immunology,Zhengzhou 450002,China;4.Zhengzhou University,Zhengzhou 450001,China);Preparation and Identification of Hyperimmune Rabbit-anti-CSFV Serum[J];Journal of Henan Agricultural Sciences;2009-05
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 LI Ya-li1,2,3,ZHOU Jie1,2,LI Jing-xi1,2,LI Qiao-mu3,4,WANG Li3,CHAI Shu-jun3,WANG Xuan-nian1,2,3*(1.Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China;2.Henan Institute of Science and Technology,Xinxiang 453003,China;3.Henan Provincial Key Laboratory of Animal Immunology,Zhengzhou 450002,China;4.Zhengzhou University,Zhengzhou 450001,China);Preparation and Identification of Hyperimmune Rabbit-anti-CSFV Serum[J];Journal of Henan Agricultural Sciences;2009-05
2 ZHU Wen-hao1,2,ZHANG Yi-fang2,XU Yin-di1,WANG Zhi-fang1,ZHANG Yu-yang1,GUO Cheng-liu1 (1.Institute of Animal Husbandry and Veterinary Research,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China);Cloning and Expression of E2 Gene of Classic Swine Fever Virus[J];Journal of Henan Agricultural Sciences;2009-09
3 YANG Su-zhen1,2,ZHANG Gai-ping1,BAO Deng-ke1,2,QIAO Song-lin1,2,WAN Bo1,2,FAN Jian-ming1,ZHI Ai-min1,LI Xue-wu1(1.Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agriculture Sciences,Zhengzhou 450002,China;2.College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450096,China);Prokaryotic Expression and Activity Analysis of VP1 Gene of Foot-and-Mouth Disease Virus Serotype O[J];Acta Agriculturae Boreali-Sinica;2009-06
4 WU Cheng-ye1,2,WANG Ai-ping2,HAO Hui-fang1,SHI Ping-ling2,YOU Lei-ming1,LI Pei-pei2,ZHANG Gai-ping1(1.Key Laboratory of Animal Immunology of the Ministry of Agriculture,Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agriculture Sciences,Zhengzhou 450002,China;2.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,China);Phylogenetic Analysis and Prokaryotic Expression of NP Gene from Subtype H5N1 of Avian Influenza Virus in Escherichia coli[J];Acta Agriculturae Boreali-Sinica;2010-03
5 YANG Yan-yan,WANG Li,YANG Ji-fei,ZHI Yu-bao,TENG Man,DENG Rui-guang,XIAO Zhi-jun,ZHANG Gai-ping(Key Laboratory for Animal Immunology of the Ministry of Agriculture,Henan Provincial Key Laboratory for Animal Immunology,Henan Academy of Agriculture Sciences,Zhengzhou 450002,China);Prokaryotic Expression,Purification and Identification of Rabies Virus Glycoprotein Extracellular Domain Gene[J];Acta Agriculturae Boreali-Sinica;2010-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 BAI Li, QIAN Jin-hu, WANG Jin (Department of Microbiology& Immunology,Dali Medical College,Dali 671000, China);Purification of Mouse IgG from Ascites Fluid and Serum by Caprylic Acid and Ammonium Sulfate[J];Journal of Dali Medical College;2000-04
2 YANG Yan-yan,ZHANG Gai-ping,XIAO Zhi-jun,YANG Ji-fei,CHAI Shu-jun,FENG Chun-hua,WANG Xuan-nian(Institute of Biotechnology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China);The Establishment of Hybridoma Cell Lines Secreting Monoclonal Antibodies to CSFV[J];Acta Agriculturae Boreali-Sinica;2006-05
3 ZANG Jin-can,FAN Guo-yan,QIAO Hong-xing,LIU Wei-min(Zhengzhou College of Animal Husbandry Engineering,Zhengzhou 450011,China);Comparative Research on Detecting Swine Fever Virus by RT-PCR and Rabbit-cross Test[J];Journal of Henan Agricultural Sciences;2007-01
4 YUAN Jian-qin1,GAO Bin-zhan2(1.College of Life Science,Shanxi Agricultural University,Taigu 030801,China;2.Shanxi Lucker Stock Corporation,Taigu 030800,China);The Analysis of the Entire Gene Sequence of H9N2 Avian Influenza Virus[J];Journal of Henan Agricultural Sciences;2007-06
5 LI Xue-wu1,ZHANG Gai-ping1,GUO Qi-xiang2(1.Henan Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 2.Henan Entry-exit Inspection and Quarantine Bureau,Zhengzhou 450003,China);Studies on Biological Characteristics of Classical Swine Fever Virus[J];Journal of Henan Agricultural Sciences;2007-10
6 Sui Guangchao. Hu Meihao.(Deparrment of Biology, Peking University.Beijing 100871).;Factors Influencing the Expression of ForeignGenes in E. coli.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1994-02
7 LONG Jin-xue WANG Qu-zhi LU Jian-hong LIU Yu-liang LIU Xiu-fan~*(Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China);Cloning of full-length genes of H5N1 subtype Avian influenza virus strain A/duck/Shandong/093/2004 and analysis of the sequences[J];Acta Microbiologica Sinica;2005-05
8 WU Chun-feng1,3,MA Zhong-sen1,WANG Xiu-li2,YANG Jun-ling1,YANG Yang1,ZHANG Yue1,TUMEN Wu-li-ji1,QIAO Jun-hua11Department of Respiratory Diseases,2Department of Hematic Diseases,the Second Clinical College,Jilin University,Changchun 130041,3Department of Respiratory Diseases of Wards for Senior Cad Res,the Peoples' Hospital of Jilin Province,Changchun 130021,China;Preparation and identification of monoclonal antibodies against human DcR3[J];Chinese Journal of Cellular and Molecular Immunology;2008-02
9 Zhou Guangsen, Wang Xueling, Miao Zhilan, Sun Lijuan (Department of Animal Science, Tianjin Agricultural College,Tianjin 300381);PPA ELISA TEST IN THE DIAGNOSIS OF SUBCLINICAL HOG CHOLERA INFECTION[J];ACTA VETERINARIA ET ZOOTECHNICA SINICA;1997-04
10 XIE Zhi qin,XIE Zhi xun,LIAO Min,DENG Xian wen,PENG Yao shan, LIU Jia bo,TANG Xiao fei,HE Jing min,TANG Cui yin (Guangxi Veterinary Research Institute, Nanning 530001, China,);Establishment and application of reverse transcriptase polymerase chain reaction for detection of hog cholera virus[J];Animal Husbandry & Veterinary Medicine;2002-11
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved