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《Journal of Henan Agricultural Sciences》 2009-12
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SNP Detection of Intron 2 of PRL Gene and Its Associations with Egg-laying Traits in Yangzhou Geese

ZHANG Gao-na1,HAN Hou-ming2,WANG Zhi-yue1,YANG Hai-ming1,ZHANG Kang-ning2 (1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.Changzhou Lihua Livestock and Poultry CO.Ltd.,Changzhou 213168,China)  
PRL is an important candidate gene,which influences the avian species'broodiness and egg performance.The egg performance of geese is low and has breeding seasonality.Therefore,in order to increase their egg performance,the single nucleotide polymorphism(SNP) sites of PRL gene intron 2 were detected in Yangzhou geese by PCR-SSCP method and the correlation analysis between the polymorphism of PRL gene intron 2 and egg-laying traits was carried out.The results showed that there is a mutation in intron 2 of PRL gene,GA insertion/deletion at the site of 32 and 33 nucleotides,which resulted in 3 genotypes(AA,BB and AB).The result showed that allele frequency of A and B are 0.5863 and 0.4137,and the genotype frequency of AA,BB and AB are 0.4619,0.2487 and 0.2894,respectively.Analysis showed that the distribution of the three genotypes was inconsistent with the Hardy-Weinberg equilibrium.Mutation of intron 2 of Yangzhou geese have no significant effect on the egg production and the age at first egg,but in egg production and the age at first egg of genotype are BBAAAB.
【Fund】: 江苏省科技支撑计划(BE2009351)
【CateGory Index】: S835
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