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《Journal of Henan Agricultural Sciences》 2018-07
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Applications of Virus-induced Gene Silencing for Analysis of Gene Function in Solanaceae Species

QU Ling;LI Yanlong;AN Wei;JIAO Enning;ZHAO Jianhua;LIU Lanying;QIN Ken;CAO Youlong;Engineering Research Institute of Wolfberry,Ningxia Acadamy of Agricultural and Forest Sciences/National Wolfberry Engineering Research Center;  
There are many important economic and medicinal plants in Solanaceae. With the success of the genome sequencing of solanaceous plants,it is urgent to identify the biological functions of a large number of unknown genes in the genome. Virus induced gene silencing( VIGS) technique is a highly efficient reverse genetics technique developed in recent 20 years to study the function of plant genes. In this paper,the advance in the functional gene mining in quality traits,growth and development,plant-fungal pathogen interactions and metabolites biosynthesis and regulation of Solanaceae species was reviewed after summarizing the principle of VIGS and the status of development and improvement of VIGS vectors. Finally,the problems and prospects of VIGS technique were discussed.
【Fund】: 国家自然科学基金资助项目(31360167);; 宁夏回族自治区自然科学基金资助项目(NZ15107);; 宁夏农林科学院对外科技合作项目(DW-X-2018012)
【CateGory Index】: Q943.2
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