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《Journal of Henan Agricultural Sciences》 2018-07
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Phylogenetic Grouping and Drug Resistance of Escherichia coli from Xinjiang Cows with Endometritis

MA Shuai;QIAO Jun;MENG Qingling;WU Yehui;WANG Xifeng;GUO Jing;CAI Kuojun;WANG Dengfeng;CAI Xuepeng;College of Animal Science and Technology,Shihezi University;Animal Disease Control and Diagnosis Center in Urumqi;Institute of Veterinary Medicine,Xinjiang Academy of Animal Science;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;  
In order to investigate the phylogenetic grouping and drug resistance of Escherichia coli from Xinjiang cows with endometritis,the E. coli strains were isolated from clinical samples,the PCR was used to detect the phylogenetic grouping and resistance genes of E. coli strains,and the susceptibility to antimicrobial agents and the ESBLs plasmids were tested and analyzed. A total of 210 E. coli strains were isolated from dairy cow samples. The phylogenetic grouping analyses showed that the proportions of group B1,A,C,E,D were 48. 1%,28. 6%,12. 9%,6. 7% and 3. 8%,respectively. The resistance analyses showedthat 210 E. coli strains possessed different degree resistance to 16 antibacterials,of which the highest rate of erythromycin was 95. 2%,followed by ampicillin 83. 3%,gentamicin 73. 8%,cephalexin 71. 9%,the lowest rate was norfloxacin 6. 2%,followed by cotrimoxazole 6. 7%,chloramphenicol 10. 0%,and doxycycline 10. 5%. The multidrug resistance range was mostly 4—10 kinds,and the drug resistance phenotype was basically consistent with the genotype. The ESBLs plasmid analyses showed that a plasmid could carry multiple drug-resistant genes,which could mediate the multi-drug resistance of strains. The results signified that the epidemic strains of E. coli in Xinjiang cow endometritis had developed severe resistance against drugs used in clinical practice and the drug resistance of the isolates was closely related to the transfer of drug-resistant plasmid.
【Fund】: 兵团中青年科技创新领军人才计划项目(2016BC001);; 国家十三五重点研发计划项目子课题(2016YFD0500900);; 新疆自治区研究生科研创新项目(XJGRI2016040);; 乌鲁木齐市科技局渝乌科技合作项目(Y161220001)
【CateGory Index】: S858.23
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