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《Journal of Henan Agricultural Sciences》 2018-07
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Effect of miR-200b on Lactation Ability of Bovine Mammary Epithelial Cells

BIAN Yanjie;HAN Jinfen;DUAN Jiangyan;Department of Biochemistry,Shanxi Normal University;  
In order to explore the effect of miR-200 b on lactating ability of bovine mammary epithelial cells( BMEC),the miR-200 b overexpression was carried out by miR-200 b mimics transfecting into the BMEC which were used as a model in vitro,the potential target genes of miR-200 b were predicted by bioinformatics. After miR-200 b overexpressing,the changes in mRNA level of the predicted target genes and the pivotal signal pathway genes related to milk composition synthesis were detected by qRT-PCR,and the cell viability and proliferation rate of BMEC were determined by CASY cell viability analyzer and Ed U assay kit,respectively. The changes of β-casein,triglyceride and lactose secretion in BMEC were analyzed by CSN2 test kit,TG test kit and lactose test kit. The bioinformatics analysis indicated that the miR-200 b binding complementary sites were located at the 3'UTR regions of Pten,Dnmt3 a and Dnmt3 b,which play important regulatory roles in the lactation of cow mammary gland. The results showed that the miR-200 b overexpression in BMEC could significantly reduce the mRNA level of Pten and significantly raise the mRNA expression of signaling pathways Akt,Srebp1,Csn2,Glut1 related to milk composition synthesis comparing with the control group,and then significantly increased the BMEC viability and prolifera-tion,and promoted the synthesis of β-casein,triglyceride and lactose. In conclusion,miR-200 b may play an important positive regulatory role in the lactation processes of dairy cows by reducing the expression of target gene Pten.
【Fund】: 国家自然科学基金项目(31701118)
【CateGory Index】: S823
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