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《Journal of Henan Agricultural Sciences》 2018-07
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Effects of Tetracyclines on the Biochemical Indexes of Carp CYP450s

LI Zaijian;LIU Wenqiang;SI Zhenshu;ZHU Mingxia;College of Agriculture,Liaocheng University;  
In order to study the effect of tetracyclines on the biochemical index of the CYP450 s,the carp liver microsomal protein content and the CYP450 activities were measured in this experiment. The carp liver microsomes were extracted by ultracentrifugation and their protein content was measured by Bradford method. The activities of CYP450 s were measured by ultraviolet spectrophotometric method. The results showed that the CYP450 content was 0. 39 ± 0. 03 nmol/mg in control group,and the 0. 22 ± 0. 02,0. 28 ± 0. 02,0. 21 ± 0. 03,0. 25 ± 0. 03 nmol/mg in treatment group( OTC,TC,CTC,DOTC); the activities of AND,ERND,AH were 1. 27 ± 0. 09,0. 85 ± 0. 04,0. 034 ± 0. 001 mol/( min · mg) in control group,in treatment groups the 0. 86 ± 0. 09,0. 79 ± 0. 05,0. 84 ± 0. 05,0. 86 ± 0. 06 nmol/( min·mg)for AND activity,the 0. 63 ± 0. 03,0. 57 ± 0. 02,0. 49 ± 0. 04,0. 52 ± 0. 02 nmol/( min·mg) for ERND activity,and the 0. 026 ± 0. 004,0. 027 ± 0. 003,0. 025 ± 0. 004,0. 028 ± 0. 004 nmol/( min·mg) for AH activity. In conclusion,tetracyclines could reduce the CYP450 content and significantly inhibit the activities of AND and ERND.
【Fund】: 山东省自然科学基金项目(2016ZRB019RS)
【CateGory Index】: S948
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