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《Acta Agriculturae Nucleatae Sinica》 1987-03
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EFFECT OF EXPLANT TREATMENT WITH GAMMA-RAYS, EMS AND SA ON SOMATIC TISSUE CULTURE IN RICE ( O ryza sativa L.)

Cheng Xiongying Gao Mingwei Liang Zhouqing (Zhejisng Agricultural University, Hangzhou)  
Dry seeds of two rice cultivars were treated with gamma-rays (0-40 kR),EMS (0-0.4M)and SA (0-4mM) and then inoculated in N6 medium containing 0.5mg/l 2,4-D,4mg/l NAA and 2mg/l 6-BA to induce callus.Growth and differentiation' of the callus treated rice seeds were observed in subsequent subcultures.Decreased callus induction percentages were observed in treatments with gamma-rays,EMS and SA,but the mu-tagenic treatments did not reduce the mean weight of primary calli except that the seeds were irradiated with gamma-rays.Moreover,up to 3-fold increase in callus growth rate was shown after treatments of the three mu-tagenic agents.Higher proportion of calli with green spots or small shoots was also seen in one cultivar treated with the three mutagens.Signif icant increase in plant regeneration capacity was found in 60-day-old calli derived from gamma-rays or EMS-treated rice seeds in one cultivar.The results suggested that the following dose or concentration can be used for explant treatment in indica rice before culture,gamma-rays.5-10kR,EMS.0.2M and SA:2-4mM.
【Fund】: 中国科学院科学基金
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【References】
Chinese Journal Full-text Database 3 Hits
1 LI Su-juan;FAN Xiu-xia;WANG Hua;SHAO Jian-feng;ZHAO Hui;XIE Fang;QIAN Qian;TAO Yue-zhi;College of Agriculture and Biotechnology,Zhejiang University;Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences;College of Life and Environmental Sciences,Hangzhou Normal University;College of Chemistry and Life Sciences,Zhejiang Normal University;State Key Lab of Rice Biology,China National Rice Research Institute;;Investigation of Regeneration and Transformation Frequencies Generated from Different Genotypes in Rice( Oryza sativa L. )[J];核农学报;2013-12
2 Shu Qingyao Wu Dianxyng Xia Yingwu Xie Jiahua Ye Abao ( Institute of Nuclear Agricultural Sciences, Zhejiang Agricultural University, Hangzhou 310029);EFFECT OF ELECTRON BEAM IMPLANTATION ON in vitro CULTURE OF YOUNG RICE PANICLE[J];核农学报;1996-02
3 Zhang Jianjun; Fan Kunhua; Zhang Zhenhua (Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences, Shanghai 201106, China. );STUDY ON VARITATION IN RICE(Oryza sativa L. )SOMACLONES FROM IN VITRO IRRADIATED SOMATOCYTES[J];上海农业学报;1994-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HEI Qian1 ZHANG Hui1 HUANG Ji-bin1 GUO Li-rong1 XU Yuan1 LI Jian-yue2 XIA Tao1 1.School of Life Science,East China Normal University,Shanghai 200062,China; 2.School of Life Science,Shanghai Normal University,Shanghai 200235,China;Overexpression of a shuffled Na~+/H~+ antiporter gene AtNHXS1 improving salt tolerance of rice(Oryza sativa L.)[J];华中农业大学学报;2012-05
2 Zhang Guiyun 1 Liu Ruru 1 Zhang Peng 2 Xu Geng 1 Zhu Jiang 2 Liang Guohua 1 Liu Qiaoquan 1* 1 Key Laboratory of Plant Functional Genomics of Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou, 225009; 2 National Center of Quality Supervision and Inspection for Toilet Articles, Yangzhou, 225009;Increase of Tocopherol Content in Endosperm by Overexpression of Homogentisate Phytyltransferase in Transgenic Rice[J];分子植物育种;2012-05
3 LI Juan,CHANG Shan-shan,LIU Feng-quan,SHAO Min (Key Laboratory of Monitoring and Management of Plant Diseases and Insects,Ministry of Education,Nanjing Agricultural University,Nanjing 210095,China;);Silencing of OsDUF500 Gene in Rice Enhances Resistance to Xanthomonas oryzae pv. oryzae[J];中国水稻科学;2012-04
4 YE Shui-feng,LI Tian-fei,LIU Guo-lan,LUO Li-jun(Shanghai Agrobiological Gene Center,Shanghai 201106,China);Production and identification of water-saving & drought-resistant transgenic indica-type rice with cry1C~* gene[J];上海农业学报;2012-02
5 YUAN Hong-xu1,LIU Wan-gou1,LIU Yue-lian2,GUO Jian-fu2,LI Yue-ren3(1 Life Science and Technology School,Zhanjiang Normal University,Zhanjiang,Guangdong 524048,China;2 Institute of Agricultural Biotechnology,Guangdong Ocean University,Zhanjiang,Guangdong 524088,China;3 Central Laboratory,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China);Inheritance analysis on sheath blight resistance and agronomic traits of transgenic rice transformed with chitinsase-gene[J];南方农业学报;2012-02
6 GAO Fang-yuan1,2,3, LU Xian-jun2,3, HE Shu-lin3,4, CHEN Xiao-juan3,4, LU Dai-hua3,4, SUN Shu-xia2,3, LI Zhi-hua2,3, LIU Guang-chun2,3, ZHANG Yi-zheng1, REN Guang-jun2,3, (1College of Life Sciences, Sichuan University, Chengdu 610041, China; 2Institute of Crop Sciences, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 3Innovation Center of Rice Regional Technology, Ministry of Agriculture, Chengdu 610066, China; 4Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China);Breeding and Characteristic Analysis of a Rice Restorer Line with Bt-Resistance Gene[J];中国水稻科学;2008-04
7 ZANG Wei 1,3 ZHANG Shu-yuan 1 ZHANG Guo-min 2 LI Zhu-gang 2 SUN Jian-qiu 1ZHANG Lan-lan 1 YAN Shan-chun 3(1.College of Life Science and Engineering,Qiqihar University,Qiqihar,Heilongjiang 161006;2.Heilongjiang Academy of Agricultural Sciences,Harbin 150040;3.College of Forestry,Northeast Forestry University,Harbin,Heilongjiang 150040);SCREENING OF RESISTANT MUTANT INDUCED BY γ-RAYS IRRADIATION TO RICE BLAST[J];核农学报;2008-03
8 YANG Yi-shan~(1,2),DENG Qi-yun~(2,*),CHEN Li-yun~1,DENG Hua-bing~1,ZHUANG Wen~2,XIONG Yue-dong~2(1 Rice Science Research Institute of Hunan Agricultural University,Changsha,Hunan 410128,China;2 China National Hybrid Rice Research and Development Center,Changsha,Hunan 410125,China);Progress in Breeding for Yield-enhancing QTL from O.rufipogon by Molecular Marker-assisted Selection[J];杂交水稻;2005-05
9 ZHENG Gui-zhao~1, HU Shi-jun~1, YANG Yue-sheng~(2*)(1.Biotechnological Institute of Dongguan,Dongguan, Guangdong 523086, China;2.Life Sciences College, South China Agricultural University, Guangzhou, Guangdong 510642, China);Evaluation of Some Culture Methods for Enhancing Plant Regeneration Ability of Rice Callus[J];杂交水稻;2005-02
10 MA Bing tian 1,2,3 , LI Ping 1,2 , ZHOU Kai da 1, ZHU Zhen 3 (1.Sichuan Agricultural Biotechnology Engineering Research Center; 2.Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China; 3.Instituteof Genet;Study on Culture Ability of Calli of Elite Indica Rice Lines[J];四川农业大学学报;2002-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WEI Xiaodong;LI Xia;Nanjing Branch of Chinese National Center for Rice Improvement, Institute of Food Crops,Jiangsu Academy of Agricultural Sciences;;Physiological Structure and Induction Characteristics of Callus in Japonica Rice[J];核农学报;2016-11
2 FENG Mingfang;CANG Jing;LU Qiuwei;ZHENG Chengcheng;ZHAO Jinmeng;LI Jiahong;QIN Lunan;YANG Wei;MENG Jing;ZHU Xiangchun;College of Life Scicece,Northeast Agricultural University;;Study on Factors of Establishment for Heilongjiang Japonica Rice Regeneration System[J];核农学报;2016-10
3 HE Xing-jian;ZENG Chong-hua;LIU Qing-bo;CHEN Fen;HU Wen-bin;WANG Zuo-ping;XIAO Guo-ying;College of Bioscience & Biotechnology ,Hunan Agricultural University;Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture ,Chinese Academy of Sciences;The Sericultural Research Institute of Hunan Province;;Preliminary Study on Transformation Efficiency of Improved Lines from Kasalath in Rice[J];现代农业科技;2016-05
4 DAI Xiaomei;SUN Zhenyuan;HAN Lei;JU Guansheng;Chinese Academy of Forestry,Research Institute of Forestry;;Callus Induction and Inhibition Effect of Citric Acid on Browning of Perennial Ryegrass Callus[J];核农学报;2015-10
5 CHEN Lei;LI Qiuyuan;YE Shenghai;JIN Qingsheng;ZHANG Xiaoming;Institute of Crops and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Science;Nanjing Institute for the Comprehensive Utilization of Wild Plants;College of Life and Environment Sciences,Shanghai Normal University;;Identification and Analysis of a Rice Lignin Monomer Synthesis Deficient Mutant[J];核农学报;2015-03
6 DAI Xiaomei;SUN Zhenyuan;HAN Lei;State Key Laboratory of Tree Genetics and Breeding/Research Institute of Forestry;;Callus Induction and Inhibition Effect of Citric Acid on Browning of Kentucky Bluegrass Callus[J];核农学报;2015-02
7 Hu Linhong;Li Tongjian;Han Xingjie;Liao Liang;Xu Lingling;School of Pharmacceutical and biological Sciences, Jiujiang University;;Study on callus inducement and differentiation from hypocotyls of “Ganzhu 3” Boehmeria nirea[J];南方林业科学;2015-01
8 LI Su-juan;FAN Xiu-xia;WANG Hua;SHAO Jian-feng;ZHAO Hui;XIE Fang;QIAN Qian;TAO Yue-zhi;College of Agriculture and Biotechnology,Zhejiang University;Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences;College of Life and Environmental Sciences,Hangzhou Normal University;College of Chemistry and Life Sciences,Zhejiang Normal University;State Key Lab of Rice Biology,China National Rice Research Institute;;Investigation of Regeneration and Transformation Frequencies Generated from Different Genotypes in Rice( Oryza sativa L. )[J];核农学报;2013-12
9 WEI Yan-Yu, ZHAO Min-An, WANG Xiao-JunXinjiang Technical Institute of Physical and Chemistry, Chinese Academy of Sciences, Urumqi 830011;Application of Plant Somaclonal Variation in Improvement of Plant Properties[J];植物生理学通讯;2004-06
10 DONG Yu ming 1,YE A bao 2 (1 Committee of Agriculture and Economy,Dinghai District,Zhoushan,Zhejiang,316000; 2 Zhoushan Institute of Agricultural Sciences,Zhoushan,Zhejiang,316041);Impacts of sweet pepper seed treated with electron current implating on germination and seedling establishment of the crop[J];浙江农业科学;2000-04
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