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《Acta Agriculturae Nucleatae Sinica》 2004-01
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ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATIONFROM LEAVES OF TRAILING PETUNIA(Petunia hybrida)

ZANG Yun-xiang 1 ZHANG Song 1 Zheng Wei-wei 1 CHEN Mei-xia 2 DA Ke-dong 1,3* WU Lu-ping 3(1. College of Horticulture, Shandong Agricultural University, Tai'an, Shandong 271018;2. Weifang Technological College, Weifang, Shandong 250086; 3. College of Horticulture, Shenyang Agricultural University,Shenyang, Liaoning 110161)  
High frequent adventitious shoot formation, plant regeneration and micro-propagation were established in trailing petunia(Petunia hybrida) leaf. Effect of different kind of hormone and its combination on adventitious shoot formation, plant regeneration and root regeneration was studied. The result showed that MS+BA 2mg/L+NAA 0.1mg/L was suitable for adventitious shoot formation from the leaf, the shoots grew well in MS+BA 0.5mg/L+IBA 0.1mg/L+GA 0.5mg/L medium, MS+IAA 0.1mg/L was the best medium for root regeneration. Turfysoil combined with perlite (1:1) was suitable for the growth of transplanted plantlet.
【CateGory Index】: S681
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