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《Journal of Nuclear Agricultural Sciences》 2018-02
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Effects of ~7Li Ion Beam Irradiation on Grain Shape,Quality and Genetic Variation in Barley

LI Bo;WANG Rong;ZHAO Linshu;LIU Luxiang;ZHANG Wenying;XU Yanhao;Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture,Yangtze University;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences;  
Dry seeds of barley were implanted by 5 different doses( 10,20,30,40,50 Gy) of ~7Li ion beam to investigate the grain shape,quality and genetic variation in M_5 generation. Near infrared spectroscopy( NIRS) was used to assess quality of 4 184 M_5 mutants. Automatic seed analyzer was used to measure the grain shape of the mutants.Fourteen ISSR primers were used to detect the genetic variations in the mutants. The results showed that each dose ~7Li ion beam could induce a broad variation in grain shape and quality. The highest variation coefficient of grain shape was obtained with 30 Gy dose treatment and the highest variation coefficient of grain quality was obtained with 50 Gy dose treatment. The maximum grain circumference variation increased 29. 15% ,reached to 36. 68 mm,the largest variation coefficient is 3. 59% . The maximum grain length variation increased 33. 39% ,reached to 15. 74 mm,the largest variation coefficient is 3. 88% . The maximum grain width variation increased by 21. 29% ,reached to 5. 07 mm,the largest variation coefficient is 6. 34% . The protein content in the mutant lines ranged from 25. 40% to 8. 51% ,the highest content increased 90. 55% ,and the lowest declined 36. 16% . The highest starch content in the mutant lines was 57. 04,increased 13. 63% and the lowest content was only 39. 62% ,declined 21. 08% . ISSR analysis showed the genetic variation frequency was 1. 92% . A novel band was detected in the 30 Gy treatment group,and a missing band was detected in the 50 Gy treatment group. This study has created novel germplasms for the improvement of barley grain shape and quality,also provided insight into using ~7Li ion beam on barley mutation breeding.
【Fund】: 国家重点研发计划(2016YFD0102101)
【CateGory Index】: S512.1
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