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《Journal of Nuclear Agricultural Sciences》 2018-02
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Cloning of Gene PmNCED1 and Its Response to PEG Stress in Common Millet

WANG Ruiyun;YANG Yang;WANG Haigang;CHEN Ling;WANG Lun;LU Ping;LIU Minxuan;QIAO Zhijun;College of Agriculture,Shanxi Agricultural University;Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture/Institute of Crop Germplasms Resources of Shanxi Academy of Agricultural Sciences;Institute of Crop Science,Chinese Academy of Agricultural Sciences;  
To explicit the characteristics of 9-cis-epoxycarotenoid dioxygenase( NCED) gene and its response to drought stress,a NCED from common millet Huangmizi,named PmNCED1,was cloned and the characters of PmNCED1 protein were predicted. Results were shown as follows. PmNCED1 contains a 1284 bp open reading frame,encodes a 428-amino acid protein. The predicted protein belongs to carotenoid cleavage dioxygenases family and NCED subfamily domain,contains one amphipathic α-helix region of AA56-75(-YARAACGLVDPSHGTGIANA-) and four conserved histidine residues. AA23-29(-ARLRQER-) and AA284-290(-TGRSTRR-) were predicted as nuclear localization signal,AA400 was predicted as nuclear export signal. The deduced amino acid sequence of PmNCED1 protein shared 77% -96% sequence identity with that of other plants. PmNCED1 protein constituted of 4 α-helixs and 25 extended strands. Quantitative Real-time PCR result showed that expression of PmNCED1 increased with PEG stress time increasing. Under PEG-6000 stress,changes of physiological indicators together with volume of bulliform cell showed significant differences( P 0. 01). It suggests that PmNCED1 was related to PEG stress. This study provide a theoretical basis for enhancing the drought tolerance of other crops such as wheat,etc.
【Fund】: 国家自然科学基金项目(31271791);; 山西省回国留学人员科研资助项目(2016-066);; 国家现代农业产业技术体系建设专项(CARS-06-13.5-A16);; 山西省重点研发计划(一般项目)(农业)项目(201603D221003-5)
【CateGory Index】: S516
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