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《Journal of Nuclear Agricultural Sciences》 2018-02
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Polyploidy Induction and Identification of Rhododendron racemosum Franch.

PENG Lyuchun;TAO Junfeng;DUAN Xiu'an;SONG Jie;XIE Weijia;GUAN Wenling;LI Shifeng;Yunnan Flower Research and Development Center/Yunnan Flower Breeding Key Laboratory/Flower Research Institute Yunnan Academy of Agricultural Sciences;College of Horticulture and Landscape,Yunnan Agricultural University;Baoshan Seed Management Station;  
In order to study mutagenic effect of colchicine on R. racemosum Franch.,aseptic seedlings of Rhododendron racemosum Franch. cultured for 15 days were used to induce and detect polyploid. The results showed that: the highest morphologic mutant rate of 32% can be obtained with the following steps: immerse seedlings in 0. 15% colchicine for 24 h,then culture the stem section without apex, and then select mutant plants among lateral axillary bud or adventitious buds differentrating from callus on the base of stem. The flow cytometer was used to identify mutant plant based on the result of morphologic screening. It is found that plantlets with thick leaf,distort leaf or thick stem are 50% probably polyploidy. The stoma of diploid is nearly round,while that of the mutant plant is oval. The short diameter and long diameter of stoma in mutant plants are larger than that in diploid plants,while the stoma number is less. Finally 141 plants are identified as tetraploid by flow cytometer. The results provide technical support and theoretical foundation for breeding new cultivar of Rhododendron by polyploidy induction.
【Fund】: 国家观赏园艺工程技术研究中心(2012FU125X10);; 云南省科技计划项目(2015BB013);; 云南省技术创新人才培养配套项目(2014-HB057);; 国家基金(31760210、31560225、31760229、31760231)
【CateGory Index】: S685.21
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