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《Journal of Nuclear Agricultural Sciences》 2018-02
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Agronomic Traits of Transgenic Cotton With High Glyphosate Resistance Conferred by Expressing the G10eve Gene

LU Guoqing;WANG Chunling;HAO Yuqiong;GUO Huiming;HUANG Yingjin;CHENG Hongmei;Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education/College of Agronomy,Jiangxi Agricultural University;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences;  
In this study,the G10 eve transgenic cotton was used to selected high resistance and stable inheritance lines in the lab and field for cultivating new glyphosate-resistant cotton. The results showed that the resistance level of transgenic cotton line 91( L91) was evidently higher than line L152( L152) after spraying different concentration of glyphosate at the cotyledon stage,and the resistance was stable. The accumulation of shikimic acid was obvious different in root,sterm and leaf,and the highest was in leaves. After the treatment of glyphosate,the expression of endogenous EPSPS increased firstly and then decreased. The relative expression level of endogenous EPSPS gene in Coker312 was significantly higher than that in transgenic lines,which further demonstrated that transgenic G10 eve gene in cotton could improve the resistance to glyphosate. In the field trials,L91 and AuR( a commercial varieties) could tolerate 20 mL·L~(-1) glyphosate. When treated with glyphosate at the seedling stage,the plant height,number of branches or number of effective bolls in L91 were increased comparing with the untreated control,while there was no significant difference in those agronomic traits of AuR compared to the control. Moreover,those agronomic traits of L91 and AuR were not inhibited when treated with glyphosate no more than 20 mL·L~(-1) during the flowering phase. These results implied that L91 could meet the requirements in production. L91 with high resistance level of glyphosate was identified and screened in this work,which provided germplasm resources for cultivating new glyphosate-resistant cotton varieties.
【Fund】: 国家转基因重大专项(2016ZX08005-004);; 中国农业科学院科技创新工程
【CateGory Index】: S562
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