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《Journal of Nuclear Agricultural Sciences》 2018-02
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Real-time PCR Assay for Detecting Potato Components in Composite Powder

YAO Xiaojing;ZHANG Hong;ZHANG Chunjiang;HU Honghai;YU Caihe;DAI Xiaofeng;Comprehensive Key Laboratory of Agro-products Processing,Ministry of Agriculture/Institute of Agro-products Processing Science and Technology,Chinese Academy of Agricultural Sciences;Institute of Staple Food Processing Technology,Chinese Academy of Agricultural Sciences;Academy of Food and Nutrition Health,Chinese Academy of Agricultural Sciences;  
Specific primers were designed using a portion of the potato beta-solanine/beta-chaconine rhamnosyltransferase( Sgt3) gene to establish a method for the quantification of potato components in composite powder by Real-time PCR,in order to monitor product quality. And thermal degradation of potato genes during heating was investigated. By comparing the amplification effect of 4 pairs of primers StLs,scuF,UGPase and Sgt3 in RTFQ PCR test,we finally determined that primer Sgt3 could amplify potato DNA specifically,and the standard curve with significant linear relationship could be obtained. The results was positive reaction when the potato concentration was higher than 2% by using Real-time PCR method. The roast potato powder-wheat powder got the linear equation with R~2= 0. 9834 and the composite powder with 50% roast potato powder( Ro PP)( w/w) was proved to 50. 75% potato( w/w). The raw potato powder-wheat powder got the linear equation with R~2= 0. 9945 and the powder with 45% raw potato powder( Ra PP) was proved to 44. 42% potato( w/w). The DNA of raw potato powder stored at 100℃ more than 10 h was degraded seriously,and not suitable for amplification. Real time fluorescence quantitative PCR technique can identify the potato-origin components. It can achieve the quantization analysis for a single known component sample,and provide the technical support for the quality supervision of potato staple food products.
【Fund】: 中国农业科学院科技创新工程协同创新任务(CAAS-XTCX2016005);; 农业公益性行业科研专项(201503001-2);; 农业部马铃薯主食产品工艺研发与市场推广试点项目(10162130106221909)
【CateGory Index】: O657.3;TS215
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