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《Journal of Nuclear Agricultural Sciences》 2018-02
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Study on the Separation and Purification,Structure Identification and in Vitro Activity of Hericium erinaceus

HE Jinzhe;FAN Peng;SUN Peilong;Department of Food Processing and Security,Ocean College,Zhejiang Unversity of Technology;  
In order to study the structure and bioactivity of the functional components of Hericium erinaceus,the mycelium of Hericium erinaceus was isolated and purified with its structure identified. Its antibacterial activity,antitumor activity,ability to stimulate growth factor of nerve cells were also tested. The crude terpene substance was extracted from the extraction of ethanol reflux,petroleum ether,and ethyl acetate. The component Fr-3-1 was isolated and purified by silica gel column chromatography and semi-preparative high performance liquid chromatography of the crude terpene,which was identified as the analogues of erinacine A via infrared spectroscopy and mass spectrometry. The antibacterial activity of Fr-3-1 was determined by filter paper method,and its antitumor activity was measured by MTT assay.Enzyme linked immunoassay was used to evaluate the ability to stimulating the growth of nerve cells. The results indicated that Fr-3-1 had remarkable antibacterial activity against Staphylococcus aureus and had certain inhibitory effect on MGB-523 cancer cells with the MIC respectively of 0. 781 mg·mL~(-1) and 453. 14 μg·mL~(-1). When the concentration of Fr-3-1 was 100 μg·mL~(-1),the secretion of growth factor of rat astrocytic cells was 46. 63 pg·mL~(-1),which was significantly higher than that of epinephrine. All of these results provided experimental evidences that Heriacium erinaceus can act as alternative drugs for the Alzheimer and neurasthenia symptoms diseases,and can promote the development and application as healthy food.
【Fund】: 国家高技术研究发展计划(863计划)项目(2014AA022205)
【CateGory Index】: TQ461;TS218
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