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《Journal of Nuclear Agricultural Sciences》 2018-02
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Enzymatic Extraction of Polyphenols From Pineapple Peel and Stability Evaluation of the Extracted Polyphenols

TANG Xiao;SUN Yangying;PAN Daodong;DAI Yujun;ZHENG Lanting;CAO Jinxuan;School of Marine Sciences,Ningbo University;Hubei Engineering University;  
In order to investigate extraction conditions of pineapple peel polyphenols and improve their preservation and application,single factorial experiments and quadratic regression orthogonal design were employed to optimize enzymatic extraction of polyphenols from pineapple peel with cellulase in this paper. The results showed that yield of total polyphenols from pineapple peel was highest( 3. 71% ) at optimal extraction conditions as follows: cellulase concentration of 7. 00 U·mg~(-1),pH value of 4. 8,liquid-solid ratio of 36∶ 1,enzymatic hydrolysis temperature of 39℃,extraction time of 65 min and 4 times extraction. The obtained yield of pineapple peel total polyphenols was consistent with that of the predicted value( 3. 82% ). Stability of the extracted pineapple peel polyphenols was also evaluated in terms of thermal stability. The extracted polyphenols were relatively stable at temperature less than 70℃ and dark conditions were beneficial to their stability. Oxidant such as hydrogen peroxide was found to have a destructive effect on the polyphenols. However,reductant such as sodium sulfite had little impact on the stability of pineapple peel polyphenols. These results provided a theoretical basis for the application of the polypheonls production.
【Fund】: 浙江省重大科技专项重大农业项目(2014C02020)
【CateGory Index】: R284.2
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