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《Journal of Chinese Practical Diagnosis and Therapy》 2013-05
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Comparison of different diagnostic methods for rapid detection of human parvovirus B19 infection

XU Dong-liang,ZHANG Guo-cheng,NIE Xiao-jing,CHENG Sheng-quan,SUN Xin, LI Zhi-hong,YANG Xiao-lei,LV Xiang-ping(Department of Pediatrics,Xijing Hospital of the Fourth Military Medical University,Xi'an 710032,China)  
Objective To compare and evaluate the ELISA method constructed by our laboratory with human parvovirus B19(B19),German ELISA Kit and PCR technique for detecting B19 infection.Methods Our own ELISA method was modified by being coated with B19 VP1 unique(VP1u) recombinant protein.IgM and IgG were detected with the modified ELISA method and German B19 ELISA Kit in 215 blood samples.B19 DNA was detected with PCR technique.The sensibility,specificity and accuracy were analyzed and compared.Results The optimal quantity of coated VP1u protein was 25 ng per well,and the optimal dilution of sample was 1∶200.The sensibility,specificity and accuracy were 79.07%,87.18% and 94.89% for B19 IgM,and 88.46%,90.79% and 93.53% for B19 IgG compared with German B19 ELISA Kit,and were 90.32%,77.77% and 95.71% for B19 IgG compared with PCR technique.Conclusion Our own modified ELISA method has good concordance with German B19 ELISA Kit and PCR technique,and can be used as a routine clinical diagnosis method for B19 infection.
【Fund】: 国家自然科学基金(81070543)
【CateGory Index】: R440
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